应用实时聚合酶链反应区分厄瓜多尔的致病性和非致病性 。
Use of Real-Time Polymerase Chain Reaction to Differentiate between Pathogenic and the Nonpathogenic in Ecuador.
机构信息
Carrera de Medicina, Instituto de Biomedicina, Universidad Central, Quito, Ecuador.
Laboratorio Clinico, Hospital General Docente Calderón MSP, Quito, Ecuador.
出版信息
Am J Trop Med Hyg. 2019 Jan;100(1):81-82. doi: 10.4269/ajtmh.17-1022.
Abstract
Microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis of intestinal parasites. Recently, polymerase chain reaction (PCR) has been approved by the World Health Organization as the method of choice for the diagnosis of . Of the 106 stool samples collected from the Esmeraldas and Pichincha provinces of Ecuador, all (100%) were positive for / by light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69.8%) were positive for and only three (2.8%) were positive for . Some 29 (27.4%) samples were negative for the presence of either or , this may be due the presence of , which is morphologically identical to / and not specifically targeted by the RT-PCR used. These results indicate the necessity of reevaluating the epidemiology of amebiasis in Ecuador as the prominent species found are nonpathogenic.
摘要
粪便样本的显微镜检查一直被认为是肠道寄生虫诊断的“金标准”。最近，聚合酶链反应（PCR）已被世界卫生组织批准为首选方法，用于诊断 。在从厄瓜多尔的埃斯梅拉达斯省和皮钦查省收集的 106 份粪便样本中，所有（100%）经光镜检查均为 阳性，而使用实时 PCR（RT-PCR）DNA 扩增，74 份（69.8%）为 阳性，仅 3 份（2.8%）为 阳性。约 29 份（27.4%）样本未检出 或 ，这可能是由于存在 ，其形态与 RT-PCR 所用的 相同，而非其特定靶标。这些结果表明，有必要重新评估厄瓜多尔的阿米巴病流行病学，因为发现的主要物种是非致病性的。
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