Otani T, Mori R
Arch Virol. 1987;96(1-2):1-15. doi: 10.1007/BF01310986.
Previously, we demonstrated that Ia+ epidermal cells (EC) have herpes simplex virus (HSV) antigen-presenting capacity in vitro and play an important role in resistance to HSV infection in vivo. In the present study, we investigated the effects of in vivo ultraviolet (UV) irradiation of the skin on the HSV-immunity function of EC both in vitro and in vivo and on the pathogenesis of HSV infection. Immune T cells cultured with EC and HSV antigen showed a proliferative response in vitro. Exposure of the skin to UV light 1 to 3 days before preparation of EC resulted in dose-dependent impairment of this proliferation. This UV-induced impairment of the accessory cell function of EC was accompanied by a parallel reduction of the number of Ia+ EC. We also transferred these EC-stimulated T cells to intracutaneously infected nude mice. Immune T cells stimulated with EC obtained from irradiated mice did not effectively clear HSV and allowed development of zosteriform skin lesions. In contrast normal-EC-stimulated immune T cells completely prevented the formation of a zosteriform rash. In addition, mice irradiated with UV on shaved midflank skin 2 days before intracutaneous inoculation of HSV showed increased severity of infection and a higher incidence of latency compared with control mice. These studies indicate that in vivo UV irradiation of the skin abrogates the immune function of EC both in vitro and in vivo, and affects HSV pathogenesis. The implication of our results for the better understanding of the effect of UV on acute and recurrent HSV infections is discussed.
此前，我们已证明Ia⁺表皮细胞（EC）在体外具有单纯疱疹病毒（HSV）抗原呈递能力，且在体内对HSV感染的抵抗力中发挥重要作用。在本研究中，我们调查了皮肤的体内紫外线（UV）照射对EC在体外和体内的HSV免疫功能以及HSV感染发病机制的影响。用EC和HSV抗原培养的免疫T细胞在体外显示出增殖反应。在制备EC前1至3天对皮肤进行UV照射会导致这种增殖出现剂量依赖性损伤。这种UV诱导的EC辅助细胞功能损伤伴随着Ia⁺EC数量的相应减少。我们还将这些由EC刺激的T细胞转移到经皮内感染的裸鼠体内。用从受照射小鼠获得的EC刺激的免疫T细胞不能有效清除HSV，并导致带状疱疹样皮肤病变的发展。相比之下，正常EC刺激的免疫T细胞完全阻止了带状疱疹样皮疹的形成。此外，在皮内接种HSV前2天对剃毛的胁腹中部皮肤进行UV照射的小鼠与对照小鼠相比，感染严重程度增加且潜伏发生率更高。这些研究表明，皮肤的体内UV照射在体外和体内均消除了EC的免疫功能，并影响HSV发病机制。我们讨论了研究结果对于更好理解UV对急性和复发性HSV感染影响的意义。
