Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang, 310008, China.
Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang, 310008, China.
Plant Physiol Biochem. 2018 Dec;133:107-115. doi: 10.1016/j.plaphy.2018.10.029. Epub 2018 Oct 29.
Tea is a non-alcoholic beverage with many benefits to human health and thereby widely consumed in the world. It contains plenty of secondary metabolites and tea catechins are the characteristic compounds. To further elucidate the biosynthetic and regulatory mechanisms of catechins in tea, high performance liquid chromatography (HPLC) and transcriptome analysis were performed in tea seedlings of different growth stages. A combined method of differential expression and correlation analysis was then conducted. The results showed that the order of total catechin (TC) contents was leaves > stems > roots, irrespective of growth stages. For transcriptome analysis, a total of 355.81 million clean reads were generated and mapped to the referencing tea genome. Further real time PCR analysis of 18 selected genes confirmed RNA-Seq results. A total of 7 structural genes and 35 transcription factors (TFs) were identified to be significantly correlated with TC changes. Among them, three TFs homologous to ANL2, WRKY44 and AtMYB113 might play key roles in catechin regulation. The de novo transcriptome data of different organs in tea seedlings provided new insights into the biosynthetic and metabolic pathways of catechins.
茶是一种对人体健康有益的非酒精饮料，因此在世界范围内广泛饮用。它含有丰富的次生代谢产物，儿茶素是其特征化合物。为了进一步阐明儿茶素在茶树中的生物合成和调控机制，对不同生长阶段的茶树幼苗进行了高效液相色谱（HPLC）和转录组分析。然后采用差异表达和相关性分析相结合的方法。结果表明，不论生长阶段如何，总儿茶素（TC）含量的顺序为叶片>茎>根。对于转录组分析，共生成 35581 万条清洁reads，并映射到参考茶树基因组。进一步对 18 个选定基因进行实时 PCR 分析，验证了 RNA-Seq 的结果。共鉴定出 7 个结构基因和 35 个转录因子（TFs）与 TC 变化显著相关。其中，三个与 ANL2、WRKY44 和 AtMYB113 同源的 TF 可能在儿茶素调控中发挥关键作用。茶树幼苗不同器官的从头转录组数据为儿茶素的生物合成和代谢途径提供了新的见解。
