Abd-Elhamid Yasser A, Eltanawy Refaat M, Fawzy Rasha M, Fouad Nehad A, Atlm Ann M
Department of Rheumatology & Rehabilitation & Physical Medicine, Faculty of Medicine, Benha University, Benha, Egypt.
Department of Microbiology & Immunology, Faculty of Medicine, Benha University, Benha, Egypt.
Egypt J Immunol. 2017 Jan;24(1):67-78.
CD64 is a type of integral membrane glycoprotein known as FC receptor that binds monomeric IgG-type antibodies with high affinity. It is more commonly known as FC gamma receptor 1 (FC?R1) and it is expressed on monocytes surface. The goal of this study was to investigate the association of CD64 expression on the surface of peripheral blood monocytes of systemic lupus erythematosus patients with disease activity, and lupus nephritis. 30 SLE patients were enrolled into this study. They were subdivided into: 15 SLE patients with lupus nephritis and 15 SLE patients without lupus nephritis. In addition,Together with 25 age and sex matched healthy volunteers were enrolled as controls group. Disease activity was defined by SLE Disease Activity Index (SLEDAI) score and the renal Systemic Lupus Erythematosus Disease Activity Index (rSLEDAI) score. Surface expression of CD64 on peripheral blood monocytes was evaluated by Flowcytometry. Renal biopsies of Lupus nephritis patients was obtained and evaluated using the International Society of Nephrology/Renal Pathology Society (ISN/RPS) classification scheme. There was a statistically significant difference in surface expression of CD64 on circulating monocytes (P > 0.001) in SLE patients with nephritis especially those with class II/III as compared to SLE without nephritis and healthy controls. The mean fluorescent intensity of CD64 staining correlated positively with markers of systemic inflammation, lupus nephritis, SLEDAI and rSLEDAI scores. In conclusions, surface expression of CD64 on circulating monocytes reflects systemic inflammation, renal injury and could be used as a rapid approach and good biomarker for disease activity and lupus nephritis in SLE patients.
CD64是一种被称为Fc受体的整合膜糖蛋白，它能以高亲和力结合单体IgG型抗体。它更 commonly被称为Fcγ受体1（FcγR1），并表达于单核细胞表面。本研究的目的是探讨系统性红斑狼疮患者外周血单核细胞表面CD64表达与疾病活动度及狼疮性肾炎的相关性。30例SLE患者纳入本研究。他们被分为：15例有狼疮性肾炎的SLE患者和15例无狼疮性肾炎的SLE患者。此外，25名年龄和性别匹配的健康志愿者作为对照组。疾病活动度由SLE疾病活动指数（SLEDAI）评分和肾脏系统性红斑狼疮疾病活动指数（rSLEDAI）评分定义。通过流式细胞术评估外周血单核细胞表面CD64的表达。获取狼疮性肾炎患者的肾活检组织，并使用国际肾脏病学会/肾脏病理学会（ISN/RPS）分类方案进行评估。与无肾炎的SLE患者和健康对照相比，有肾炎的SLE患者尤其是II/III级患者循环单核细胞表面CD64的表达存在统计学显著差异（P>0.001）。CD64染色的平均荧光强度与全身炎症、狼疮性肾炎、SLEDAI和rSLEDAI评分的标志物呈正相关。总之，循环单核细胞表面CD64的表达反映全身炎症、肾损伤，可作为SLE患者疾病活动度和狼疮性肾炎的快速检测方法及良好生物标志物。
