Werner U
Z Klin Chem Klin Biochem. 1975 Aug;13(8):341-9.
Commercially available columns for ion exchange chromatography were used for the separation of catecholamines in urine. The estimation of catecholamines was performed fluorimetrically by a new trihydroxyindole method. The fluorescence of adrenaline or noradrenaline was enhanced, in comparison with other methods, by the combined application of boric acid, copper-ions, mercaptoethanol and final reacidification. The fluorophores are stable: The loss of fluorescence of adrenolutin amounted to 22% during 180 minutes, and there was no loss of noradrenolutin. For the differentiation of amines, adrenaline was oxidized at pH 2.85 and noradrenaline at pH 7. Precision, accuracy, sensitivity and specifity fulfilled the criteria of analysis. The normal values, determined in a collective of 17 healthy persons, were adrenaline (x +/- s) 44.8 +/- 16.9 nmol/24 h and noradrenaline (x +/- s) 224.0 +/- 68.0 nmol/24h. The simultaneous application of alpha-methyldopa (2 g/day) in 13 patients with primary hypertension did not disturb the fluorimetric estimation of catecholamines.
使用市售的离子交换色谱柱分离尿液中的儿茶酚胺。采用一种新的三羟基吲哚法通过荧光法测定儿茶酚胺。与其他方法相比，通过联合应用硼酸、铜离子、巯基乙醇并最终再次酸化，肾上腺素或去甲肾上腺素的荧光得以增强。荧光团很稳定：肾上腺色素的荧光在180分钟内损失了22%，而去甲肾上腺色素没有损失。为了区分胺类，肾上腺素在pH 2.85时被氧化，去甲肾上腺素在pH 7时被氧化。精密度、准确度、灵敏度和特异性均符合分析标准。在17名健康人的群体中测定的正常值为：肾上腺素（x±s）44.8±16.9 nmol/24小时，去甲肾上腺素（x±s）224.0±68.0 nmol/24小时。13例原发性高血压患者同时应用α-甲基多巴（2 g/天）并未干扰儿茶酚胺的荧光测定。
