Ueda E, Yoshida N, Nishimura K, Joh T, Antoku S
Clin Chim Acta. 1977 Nov 1;80(3):447-53. doi: 10.1016/0009-8981(77)90137-1.
Semi-automated measurement of urinary catecholamines, specific for dopa, noradrenaline, adrenaline and dopamine and other metabolites has been developed. Steps included alumina extraction, separation by high-speed ion-exchange column chromatography and fluorescent development by trihydroxyindole (THI) method without interference by other fluorescent substances. Recovery, reproducibility and sensitivity were satisfactory. alpha-Methyldopa, a metabolite of alpha-methyldopa, and isoproterenol were separated completely without interfering with the measurement of noradrenaline, adrenaline and dopamine.
已经开发出用于对多巴、去甲肾上腺素、肾上腺素、多巴胺及其他代谢产物进行特异性检测的尿儿茶酚胺半自动测量方法。步骤包括氧化铝提取、通过高速离子交换柱色谱法分离以及采用三羟基吲哚（THI）法进行荧光显色，且不受其他荧光物质干扰。回收率、重现性和灵敏度均令人满意。α-甲基多巴（α-甲基多巴的一种代谢产物）和异丙肾上腺素能被完全分离，不干扰去甲肾上腺素、肾上腺素和多巴胺的测量。
