Wainwright R S
Ann Clin Biochem. 1978 Mar;15(2):93-101. doi: 10.1177/000456327801500122.
A semiautomated method is presented for the determination of adrenaline and noradrenaline by a combination of the trihydroxyindole procedure for total catecholamines and a new procedure for the specific direct determination of adrenaline without interference from noradrenaline at physiological levels. This new procedure involves the reaction of adrenaline with alkaline thiosemicarbazide to produce a fluorophor of acceptable stability for use in automated procedures. Its structure is as yet unknown. The within-batch standard deviation for the trihydroxyindole and alkaline thiosemicarbazide procedures respectively were 0.005 mumol/24h at a concentration of 0.368 mumol/24h and 0.012 mumol/24h at a concentration of 0.133 mumol/24h. The between-batch standard deviations of the two procedures were respectively 0.018 mumol/24h at a concentration of 0.388 mumol/24h and 0.015 mumol/24h at a concentration of 0.111 mumol/24h. Normal ranges for urine are presented for 24 healthy ambulant laboratory staff. The total catecholamine concentration (calculated as adrenaline) was 0.452 +/- 0.069 mumol/24/ (SD), range 0.349-0.616 mumol/24h. Using the alkaline thiosemicarbazide procedure, the normal range which was determined for adrenaline was 0.100 +/- 0.054 mumol/24h, range 0.022-0.207 mumol/24h. Noradrenaline calculated by difference was 0.355 +/- 0.081 mumol/24h, range 0.195-0.497 mumol/24h.
本文介绍了一种半自动方法，用于测定肾上腺素和去甲肾上腺素。该方法结合了用于测定总儿茶酚胺的三羟基吲哚法和一种新的特定直接测定肾上腺素的方法，该方法在生理水平下不受去甲肾上腺素干扰。这种新方法涉及肾上腺素与碱性硫代氨基脲反应，生成一种稳定性可接受的荧光团，用于自动化程序。其结构尚不清楚。三羟基吲哚法和碱性硫代氨基脲法的批内标准差分别为：浓度为0.368μmol/24h时为0.005μmol/24h，浓度为0.133μmol/24h时为0.012μmol/24h。两种方法的批间标准差分别为：浓度为0.388μmol/24h时为0.018μmol/24h，浓度为0.111μmol/24h时为0.015μmol/24h。给出了24名健康门诊实验室工作人员的尿正常范围。总儿茶酚胺浓度（以肾上腺素计算）为0.452±0.069μmol/24/（标准差），范围为0.349 - 0.616μmol/24h。使用碱性硫代氨基脲法测定的肾上腺素正常范围为0.100±0.054μmol/24h，范围为0.022 - 0.207μmol/24h。通过差值计算得到的去甲肾上腺素为0.355±0.081μmol/24h，范围为0.195 - 0.497μmol/24h。
