Department of Food and Nutrition, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, SP, Brazil.
Division of Gastroenterology, Department of Pediatrics, BC Children's Hospital and the University of British Columbia, Vancouver, BC V5Z 4H4, Canada.
Nutrients. 2018 Nov 5;10(11):1684. doi: 10.3390/nu10111684.
Modulation of the gut microbiota through the use of probiotics has been widely used to treat or prevent several intestinal diseases. However, inconsistent results have compromised the efficacy of this approach, especially in severe conditions such as inflammatory bowel disease (IBD). The purpose of our study was to develop a personalized probiotic strategy and assess its efficacy in a murine model of intestinal inflammation. Commensal bacterial strains were isolated from the feces of healthy mice and then administered back to the host as a personalized treatment in dextran sodium sulfate (DSS)-induced colitis. Colonic tissues were collected for histological analysis and to investigate inflammatory markers such as , , , and , and the enzyme myeloperoxidase as a neutrophil marker. The group that received the personalized probiotic showed reduced susceptibility to DSS-colitis as compared to a commercial probiotic. This protection was characterized by a lower disease activity index and reduced histopathological damage in the colon. Moreover, the personalized probiotic was more effective in modulating the host immune response, leading to decreased and and increased and expression. In conclusion, our study suggests that personalized probiotics may possess an advantage over commercial probiotics in treating dysbiotic-related conditions, possibly because they are derived directly from the host's own microbiota.
通过使用益生菌来调节肠道微生物群已被广泛用于治疗或预防多种肠道疾病。然而，不一致的结果影响了这种方法的疗效，特别是在炎症性肠病（IBD）等严重情况下。我们的研究目的是开发一种个性化益生菌策略，并在肠道炎症的小鼠模型中评估其疗效。从健康小鼠的粪便中分离出共生细菌菌株，然后将其作为个性化治疗物回输给接受葡聚糖硫酸钠（DSS）诱导结肠炎的宿主。收集结肠组织进行组织学分析，并研究炎症标志物，如 、 、 、 和 ，以及作为中性粒细胞标志物的髓过氧化物酶。与商业益生菌相比，接受个性化益生菌的组对 DSS-结肠炎的易感性降低。这种保护作用的特征是疾病活动指数降低，结肠组织学损伤减轻。此外，个性化益生菌在调节宿主免疫反应方面更有效，导致 、 表达降低， 、 表达增加。总之，我们的研究表明，个性化益生菌在治疗与肠道菌群失调相关的疾病方面可能优于商业益生菌，可能是因为它们直接来源于宿主自身的微生物群。
