Makzum Somaye, Ghadam Parinaz, Ramezani Mohaddaseh
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran.
Iran J Microbiol. 2023 Apr;15(2):267-277. doi: 10.18502/ijm.v15i2.12479.
The use of probiotics as an alternative to antibiotics in poultry diets has attracted a lot of interest recently. In this context, the determination of probiotic characteristics was evaluated on several isolates obtained from Iranian poultry's gut.
Probiotic characteristics such as hemolysis activity, acid, bile, gastric juice tolerate, adhesion assay, cell surface properties (hydrophobicity, auto-aggregation and co-aggregation) and antibiotic susceptibility test were evaluated. Finally, selected isolates identified molecularly after temperature-salt tolerance and extracellular enzyme activity (amylase, protease and cellulose).
Out of 362 strains isolated from native poultry in three geographical regions of Iran, nine strains (belong to sp., sp., sp., sp., sp.) showed resistance against gastrointestinal physiological conditions, desirable surface properties, ability to adhere to epithelial intestine cell line and antibiotic susceptibility. Also, these strains were discovered to be temperature-salt tolerant but, only a small number of them were able to produce hydrolase enzymes.
According to the results, the selected strains can be introduced as native probiotic candidates for utilization in novel poultry feeds.
益生菌作为家禽日粮中抗生素的替代品，近年来备受关注。在此背景下，对从伊朗家禽肠道分离得到的几株菌株进行了益生菌特性评估。
评估了益生菌的溶血活性、耐酸、耐胆汁、耐胃液、黏附试验、细胞表面特性（疏水性、自聚集和共聚集）以及抗生素敏感性试验。最后，在评估温度盐耐受性和胞外酶活性（淀粉酶、蛋白酶和纤维素酶）后，对筛选出的菌株进行分子鉴定。
从伊朗三个地理区域的本地家禽中分离出362株菌株，其中9株（属于 属、 属、 属、 属、 属）表现出对胃肠道生理条件的抗性、良好的表面特性、黏附于肠上皮细胞系的能力以及抗生素敏感性。此外，这些菌株被发现具有温度盐耐受性，但只有少数能够产生水解酶。
根据结果，筛选出的菌株可作为新型家禽饲料中使用的本地益生菌候选菌株。
