Lin L, Dai F, Wei J J, Chen Z, Tang X Y
Department of Otorhinolaryngology Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai,200040, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Oct;32(20):1575-1579. doi: 10.13201/j.issn.1001-1781.2018.20.010.
The aim of this study is to examine whether nuocytes induced differentiation of primary T cells into Th2 cells in AR mice in vitro. A murine model of AR on the background of BALB/c was established using ovalbumin, and nuocytes were sorted and purified from the mouse nasal associated lymphoid tissue (NALT) and cultured in vitro. Then, we assessed the expression of IL-4 in these cells. Mouse peripheral blood mononuclear cells were isolated and Th2 cells and T cells were isolated for in vitro culture, and the percentage of T cells in Th2 cells was detected. Then, NALT-derived nuocyte cells cultured in vitro were added to the above-mentioned mouse T cell culture medium for co-culture. Numbers of sneezing, nasal rubbing and eosinophils in nasal lavage fluid were all enhanced in AR mice compared to normal ones. We used flow cytometry analysis to identify nuocytes from mice NALT as CD3CD4CD8CD19CD11bCD11cFcεR1 (lineage)-ICOS+, and also found that the cells expressed IL-4, and its protein and mRNA were all increased in AR mice versus normal mice. After nuocytes were co-cultured with T-cell cultures, we determined the percentage of Th2 cells in total T cells, and found that the percentage was increased significantly. nuocyte cells may induce the differentiation of primary T cells into Th2 cells in AR mice through IL-4 pathway.
本研究的目的是检测在体外卵清蛋白诱导的变应性鼻炎（AR）小鼠模型中，nuocytes是否能诱导原代T细胞分化为Th2细胞。以卵清蛋白建立BALB/c背景的AR小鼠模型，从小鼠鼻相关淋巴组织（NALT）中分选并纯化nuocytes，进行体外培养。然后，我们评估了这些细胞中白细胞介素-4（IL-4）的表达。分离小鼠外周血单个核细胞，分离出Th2细胞和T细胞进行体外培养，检测Th2细胞中T细胞的百分比。然后，将体外培养的NALT来源的nuocyte细胞加入上述小鼠T细胞培养基中进行共培养。与正常小鼠相比，AR小鼠的喷嚏次数、鼻摩擦次数和鼻腔灌洗液中的嗜酸性粒细胞数量均增加。我们用流式细胞术分析鉴定出小鼠NALT中的nuocytes为CD3CD4CD8CD19CD11bCD11cFcεR1（谱系）-ICOS+，还发现这些细胞表达IL-4，且与正常小鼠相比，其蛋白和mRNA在AR小鼠中均增加。nuocytes与T细胞培养物共培养后，我们测定了总T细胞中Th2细胞的百分比，发现该百分比显著增加。nuocyte细胞可能通过IL-4途径诱导AR小鼠原代T细胞分化为Th2细胞。
