Lin L, Dai F, Wei J J, Chen Z, Tang X Y
Department of Otorhinolaryngology Head and Neck Surgery，Huashan Hospital North of Fudan University，Shanghai，200040，China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2019 Sep;33(9):870-874;882. doi: 10.13201/j.issn.1001-1781.2019.09.018.
The study aimed to investigate the role of nuocytes in allergic rhinitis （AR） murine models. After intranasal administration of recombinant （rm） interleukin （IL）-33 in BALB/c mice, nuocytes were sorted and purified from the mouse nasal-associated lymphoid tissue （NALT）. Then, we examined the response of nuocytes to rmIL-33 in vitro. After a murine model of AR was established using ovalbumin, we adoptively transferred the cultured NALT-derived nuocytes to mice models, and determined allergic responses in them. rmIL-33 expanded nuocytes in NALT of mice compared with AR mice （=3.66, <0.01）, and increased production of IL-13 from these cells in vitro in comparison with unstimulated nuocytes （=19.90, <0.000 1）. After adoptive transfer of nuocytes, sneezing （=9.89, <0.000 1） ,numbers of eosinophils（=8.17, <0.000 1）, concentrations of IL-13 （=40.47, <0.000 1） and IL-33 （=19.89, <0.000 1） in nasal lavage fluid were all enhanced when compared with AR mice. Nuocytes promote allergic inflammation in a murine model of AR.
该研究旨在探讨nuocytes在变应性鼻炎（AR）小鼠模型中的作用。在BALB/c小鼠鼻内给予重组（rm）白细胞介素（IL）-33后，从小鼠鼻相关淋巴组织（NALT）中分选并纯化nuocytes。然后，我们在体外检测了nuocytes对rmIL-33的反应。在用卵清蛋白建立AR小鼠模型后，我们将培养的NALT来源的nuocytes过继转移到小鼠模型中，并测定其中的变应性反应。与AR小鼠相比，rmIL-33使小鼠NALT中的nuocytes扩增（=3.66，<0.01），并且与未刺激的nuocytes相比，体外这些细胞的IL-13产生增加（=19.90，<0.000 1）。nuocytes过继转移后，与AR小鼠相比，鼻灌洗液中的喷嚏次数（=9.89，<0.000 1）、嗜酸性粒细胞数量（=8.17，<0.000 1）、IL-13浓度（=40.47，<0.000 1）和IL-33浓度（=19.89，<0.000 1）均增加。Nuocytes在AR小鼠模型中促进变应性炎症。
