Huashan Hospital of Fudan University, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China.
Huashan Hospital North of Fudan University, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China.
Braz J Otorhinolaryngol. 2021 Nov-Dec;87(6):661-670. doi: 10.1016/j.bjorl.2019.12.010. Epub 2020 Feb 12.
Nuocytes play an important role in Type 2 immunity. However, the contribution of ILC2s to allergic rhinitis remains to be clearly elucidated.
To evaluate the role of nuocytes from mesenteric lymph node on allergic responses in mice.
After intraperitoneal administration of interleukin IL-25 and IL-33 to wild-type and Il17brIl1rl1 double-deficient mice, nuocytes were purified from the the nasal-associated lymphoid tissue and mesenteric lymph nodes. Then, we assessed productions of IL-5 and IL-13 in nuocytes' cultures. Finally, we adoptively transferred the mesenteric lymph node-derived nuocytes from wild-type and Il17brIl1rl1 mice to the murine model of allergic rhinitis to evaluate their roles in nasal allergic responses.
We showed that nuocytes in the mesenteric lymph nodes of wild-type mice were upregulated after application of IL-25 and IL-33, and were induced to produce IL-5 and IL-13. Numbers of sneezing and nasal rubbing as well as eosinophils were all enhanced after the adoptive transfer of wild-type nuocytes. Concentrations of IL-5, IL-13, IL-25 and IL-33 in nasal lavage fluid of allergic mice were also increased. However, nuocytes fromIl17brIl1rl1 mice did not increase sneezing and nasal rubbing and eosinophilia, and upregulate the above cytokines in the nasal lavage fluid.
The findings demonstrate that nuocytes from the mesenteric lymph nodes of wild-type mice promote allergic responses in a mouse model.
Nuocytes 在 2 型免疫中发挥重要作用。然而，ILC2s 对过敏性鼻炎的贡献仍有待阐明。
评估肠系膜淋巴结中的 nuocytes 对小鼠过敏反应的作用。
向野生型和 Il17brIl1rl1 双缺陷型小鼠腹腔内给予白细胞介素 25 和白细胞介素 33 后，从鼻相关淋巴组织和肠系膜淋巴结中纯化 nuocytes。然后，我们评估了 nuocytes 培养物中 IL-5 和 IL-13 的产生。最后，我们将来自野生型和 Il17brIl1rl1 小鼠的肠系膜淋巴结衍生的 nuocytes 过继转移到过敏性鼻炎的小鼠模型中，以评估它们在鼻过敏反应中的作用。
我们表明，IL-25 和 IL-33 应用后，野生型小鼠肠系膜淋巴结中的 nuocytes 上调，并诱导其产生 IL-5 和 IL-13。过继转移野生型 nuocytes 后，打喷嚏和鼻摩擦以及嗜酸性粒细胞的数量均增加。然而，来自 Il17brIl1rl1 小鼠的 nuocytes 并没有增加打喷嚏和鼻摩擦以及嗜酸性粒细胞增多，并且也没有增加鼻灌洗液中的上述细胞因子。
这些发现表明，来自野生型小鼠肠系膜淋巴结的 nuocytes 促进了小鼠模型中的过敏反应。
