Velasco A, Hidalgo J
Biol Cell. 1987;59(2):161-8. doi: 10.1111/j.1768-322x.1987.tb00527.x.
Two phosphatase activities, which have been reported to be associated with the Golgi apparatus in several cellular types, have been cytochemically demonstrated in rat epiphyseal cartilage. This was the case for thiamine pyrophosphatase (TPPase) which was detected in Golgi trans face cisternae and also in nascent or immature secretory granules of chondrocytes. beta-Nicotinamide adenine dinucleotide phosphatase (beta-NADPase), on the other hand, was localized mainly in the endoplasmic reticulum region of both proliferative and hypertrophic chondrocytes. Most of the beta-NADPase reaction was shown to be associated with the cytoplasmic side of the rough endoplasmic reticulum membranes and also partially dispersed throughout the cytosol background. We suggest that beta NADPase in chondrocytes could be an enzyme with different properties from that described in other secretory cells.
在几种细胞类型中，据报道有两种磷酸酶活性与高尔基体相关，现已在大鼠骨骺软骨中通过细胞化学方法证实。硫胺素焦磷酸酶（TPPase）就是这种情况，它在高尔基体反面潴泡以及软骨细胞的新生或未成熟分泌颗粒中被检测到。另一方面，β-烟酰胺腺嘌呤二核苷酸磷酸酶（β-NADPase）主要定位于增殖性和肥大性软骨细胞的内质网区域。大部分β-NADPase反应显示与粗面内质网膜的细胞质侧相关，并且也部分分散于整个细胞质背景中。我们认为软骨细胞中的β-NADPase可能是一种与其他分泌细胞中所描述的酶性质不同的酶。
