Doine A I, Oliver C, Hand A R
J Histochem Cytochem. 1984 May;32(5):477-85. doi: 10.1177/32.5.6143779.
Morphological and cytochemical changes in the Golgi apparatus and GERL of differentiating parotid acinar cells were examined in Sprague-Dawley rats from 5 days to young adult. At day 5, the Golgi apparatus consisted of 3-6 narrow saccules, with short segments of GERL lying adjacent to the trans Golgi saccule. As the glands matured, the Golgi apparatus increased in size and the saccules became broadened and fenestrated reaching a maximum from days 15-20. The saccules subsequently narrowed slightly and by day 25 resembled those seen in young adults. Numerous cisternae of GERL could be seen at the trans face during this period. While the glands were maturing, marked changes occurred in the distribution of thiamine pyrophosphatase (TPPase) activity in the Golgi saccules. In the immature cells, TPPase activity was restricted to 1 or 2 trans Golgi saccules. However, by day 10 TPPase could also be localized in immature secretory granules and in GERL-like cisternae. Unreactive segments of GERL were also present. This pattern of localization persisted until day 20, after which the TPPase activity in the GERL-like cisternae diminished gradually until by day 40 TPPase again was localized in 1-2 trans Golgi saccules and an occasional immature secretory granule. Acid phosphatase (AcPase) activity was localized primarily in lysosomes in the very young animals and increased in GERL with age up to day 15. From days 15 to 20 there was a decrease in the amount of activity seen in GERL, but from day 20 on, the AcPase activity increased until it reached that seen in young adult animals. These results indicate that the presence of TPPase activity in GERL-like cisternae and immature secretory granules may be dependent upon the developmental as well as the physiologic state of the acinar cells and lend further support to the suggestion that GERL is derived from the trans Golgi saccules.
在出生5天至成年的Sprague-Dawley大鼠中，研究了分化中的腮腺腺泡细胞高尔基体和GERL（与高尔基体相关的内质网-溶酶体系统）的形态学和细胞化学变化。在第5天，高尔基体由3-6个狭窄的扁平囊泡组成，在高尔基体反面扁平囊泡附近有短片段的GERL。随着腺体成熟，高尔基体尺寸增大，扁平囊泡变宽并出现穿孔，在第15-20天达到最大值。随后扁平囊泡略有变窄，到第25天时类似于成年动物所见。在此期间，在反面可见大量GERL的潴泡。在腺体成熟过程中，高尔基体扁平囊泡中硫胺焦磷酸酶（TPPase）活性的分布发生了显著变化。在未成熟细胞中，TPPase活性局限于1或2个高尔基体反面扁平囊泡。然而，到第10天时，TPPase也可定位于未成熟分泌颗粒和类似GERL的潴泡中。也存在无反应性的GERL片段。这种定位模式一直持续到第20天，此后类似GERL的潴泡中的TPPase活性逐渐降低，直到第40天时TPPase再次定位于1-2个高尔基体反面扁平囊泡和偶尔的未成熟分泌颗粒中。酸性磷酸酶（AcPase）活性在非常年幼的动物中主要定位于溶酶体中，并随着年龄增长在GERL中增加，直至第15天。从第15天到第20天，GERL中可见的活性量减少，但从第20天起，AcPase活性增加，直至达到成年动物中的水平。这些结果表明，类似GERL的潴泡和未成熟分泌颗粒中TPPase活性的存在可能取决于腺泡细胞的发育以及生理状态，并进一步支持了GERL源自高尔基体反面扁平囊泡的观点。
