Sinowatz F, Amselgruber W
Histochemistry. 1986;86(2):169-73. doi: 10.1007/BF00493383.
The ultrastructural localization of nicotinamide adenine dinucleotide phosphatase (NADPase) in bovine Leydig cells has been studied and compared with the pattern of thiamine pyrophosphatase (TPPase) and acid phosphatase distribution in these cells. Using beta-nicotinamide adenine dinucleotide phosphate (beta-NADP+) as substrate, a marked staining is observed in the intermediate Golgi saccules with some focal extension to the trans aspect. Cisternae on the cis side and associated vesicles yielded only slightly positive reactions. The pattern of NADPase localization is clearly different from that of TPPase which consistently stains only the trans Golgi elements. The specificity of NADPase for its substrate, beta-NADP+, was clearly demonstrated by using substrates modified in either the nicotinamide region e.g. alpha-nicotinamide adenine dinucleotide phosphate (alpha-NADP+), beta-thionicotinamide adenine dinucleotide phosphate (Thio-NADP+), in the attachment site of the monoester phosphate group to the molecule (e.g. 2' monophospho-adenosine 5'-diphosphoribose (ATP-ribose) or adenosine-5-monophosphate (5'AMP). With these substrates only weak or negative reactions were obtained in the Golgi apparatus of the bovine Leydig cell.
已对烟酰胺腺嘌呤二核苷酸磷酸酶（NADPase）在牛睾丸间质细胞中的超微结构定位进行了研究，并与硫胺素焦磷酸酶（TPPase）的模式以及这些细胞中酸性磷酸酶的分布进行了比较。以β-烟酰胺腺嘌呤二核苷酸磷酸（β-NADP +）为底物时，在高尔基中间囊泡中观察到明显的染色，向反面有一些局灶性延伸。顺面的池和相关的囊泡仅产生轻微的阳性反应。NADPase的定位模式与TPPase明显不同，TPPase始终仅对反式高尔基体元件染色。通过使用在烟酰胺区域修饰的底物，例如α-烟酰胺腺嘌呤二核苷酸磷酸（α-NADP +）、β-硫代烟酰胺腺嘌呤二核苷酸磷酸（硫代-NADP +），或在单酯磷酸基团与分子的连接位点（例如2'-单磷酸腺苷5'-二磷酸核糖（ATP-核糖）或腺苷-5-单磷酸（5'AMP））修饰的底物，清楚地证明了NADPase对其底物β-NADP +的特异性。使用这些底物时，在牛睾丸间质细胞的高尔基体中仅获得微弱或阴性反应。
