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巴蒂姆树提取物对人角质形成细胞和成纤维细胞的细胞毒性和遗传毒性分析。

Analysis of Cyto- and Genotoxicity of Barbatimão Extract on Human Keratinocytes and Fibroblasts.

机构信息

Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil.

Postgraduate Program of Gerontology, Federal University of Santa Maria, Santa Maria, RS, Brazil.

出版信息

Biomed Res Int. 2018 Oct 8;2018:1942451. doi: 10.1155/2018/1942451. eCollection 2018.

DOI:10.1155/2018/1942451
PMID:30402464
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6197002/
Abstract

Barbatimão (, Mart.) is a native Brazilian species used in traditional medicine and some commercial preparations owing to its strong wound-healing activity. However, controversy regarding its use due to safety concerns over the potential genotoxic effect of this plant remains. In order to clarify this issue, the effect of hydroalcoholic extract of barbatimão on cell viability, DNA damage, and induction of apoptosis in two commercial cell lines of keratinocytes (HaCaT) and fibroblasts (HFF-1) was evaluated. Barbatimão stem bark hydroalcoholic extract (70% ethanol) was obtained and lyophilized for subsequent use in all experiments. The main bioactive molecules quantified by HPLC were gallic acid, caffeic acid, quercetin, catechin, and epigallocatechin gallate (EGCG). Barbatimão (0.024 to 1.99 mg/mL) was found to decrease cellular mortality as compared to the control group. GEMO assay, a noncellular DNA protocol that uses HO-exposed calf thymus DNA, revealed not only a genotoxic effect of barbatimão, but also a potential genoprotective action against HO-triggered DNA fragmentation These results indicated that barbatimão at concentrations of 0.49 and 0.99 mg/mL, which are near to the levels found in commercial preparations, exerted an genoprotective effect on cells by decreasing the levels of DNA oxidation quantified by 8-hydroxy-2'-deoxyguanosine (8-OHdG) and reactive oxygen species (ROS) levels. Gene and protein apoptotic markers, quantified by qRT-PCR ( genes) and immunoassays (Caspases 3 and 8), respectively, also indicated a decrease in apoptotic events in comparison with control cells. Collectively, the results suggest that barbatimão could exert genoprotective and antiapoptotic effects on human keratinocytes and fibroblasts.

摘要

巴达维亚木（，Mart.）是一种原产于巴西的物种，因其具有很强的伤口愈合活性，被用于传统医学和一些商业制剂中。然而，由于这种植物可能具有遗传毒性作用，其使用安全性存在争议。为了解决这个问题，评估了巴达维亚木的水醇提取物对两种商业角质细胞（HaCaT）和成纤维细胞（HFF-1）系细胞活力、DNA 损伤和细胞凋亡诱导的影响。巴达维亚木茎皮水醇提取物（70%乙醇）被获得并冻干，以便随后用于所有实验。通过 HPLC 定量的主要生物活性分子有没食子酸、咖啡酸、槲皮素、儿茶素和表没食子儿茶素没食子酸酯（EGCG）。与对照组相比，巴达维亚木（0.024 至 1.99mg/mL）被发现降低了细胞死亡率。GEMO assay 是一种使用 HO 暴露的小牛胸腺 DNA 的非细胞 DNA 方案，不仅揭示了巴达维亚木的遗传毒性作用，还揭示了其对 HO 触发的 DNA 片段化的潜在遗传保护作用。这些结果表明，在接近商业制剂中发现的浓度 0.49 和 0.99mg/mL 下，巴达维亚木对细胞发挥了遗传保护作用，通过降低 8-羟基-2'-脱氧鸟苷（8-OHdG）和活性氧（ROS）水平来减少量化的 DNA 氧化水平。通过 qRT-PCR（基因）和免疫测定（Caspases 3 和 8）分别量化的基因和蛋白凋亡标记物也表明与对照细胞相比，凋亡事件减少。总的来说，这些结果表明巴达维亚木可能对人角质细胞和成纤维细胞发挥遗传保护和抗凋亡作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/8bcd81b1020b/BMRI2018-1942451.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/9ba7721b2930/BMRI2018-1942451.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/81faf6373389/BMRI2018-1942451.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/6827a3156544/BMRI2018-1942451.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/2c07c9981cfb/BMRI2018-1942451.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/8bcd81b1020b/BMRI2018-1942451.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/9ba7721b2930/BMRI2018-1942451.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/81faf6373389/BMRI2018-1942451.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/6827a3156544/BMRI2018-1942451.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/2c07c9981cfb/BMRI2018-1942451.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/326d/6197002/8bcd81b1020b/BMRI2018-1942451.005.jpg

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