Dąbrowska Natalia, Wiczkowski Andrzej
Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
Faculty of Health Sciences, The University of Bielsko-Biała, Poland.
Adv Clin Exp Med. 2017 Jan-Feb;26(1):155-166. doi: 10.17219/acem/43272.
Under homeostatic conditions, an equilibrium state between amounts of free radicals formed and their scavenging is observed. Free radicals are destructive only when present in excess. Pathological changes within cells and tissues can result from a persistent excess of free radicals. Living organisms are increasingly exposed to oxidative stress, resulting in oxidative DNA modifications. One such modification is 8-hydroxy-2'-deoxyguanosine (8-OHdG). It is considered a biomarker of oxidative stress and oxidative DNA damage. It has been found both in physiological fluids and in cells. This paper presents methods found in the literature for determining 8-OHdG expression in various kinds of biological material - blood, urine or liver homogenates. Methods for determining the biomarker expression have been grouped into direct and indirect methods, and the various levels of 8-hydroxy-2'-deoxyguanosine that can be determined by the different techniques are presented. The basic pros and cons of the various techniques are also discussed.
在稳态条件下，可观察到自由基生成量与其清除之间的平衡状态。自由基只有在过量存在时才具有破坏性。细胞和组织内的病理变化可能源于自由基的持续过量。生物体越来越多地暴露于氧化应激，导致氧化性DNA修饰。其中一种修饰是8-羟基-2'-脱氧鸟苷（8-OHdG）。它被认为是氧化应激和氧化性DNA损伤的生物标志物。已在生理体液和细胞中均发现了它。本文介绍了文献中发现的用于测定各种生物材料（血液、尿液或肝脏匀浆）中8-OHdG表达的方法。测定生物标志物表达的方法已分为直接法和间接法，并介绍了不同技术可测定的8-羟基-2'-脱氧鸟苷的不同水平。还讨论了各种技术的基本优缺点。
