Bolhuis R L, Nooyen A J
Immunology. 1977 Nov;33(5):679-87.
A receptor on T lymphocytes that can bind IgM-type antibodies, without, as well as after prior complex formation between the antibody and Esh (sheep red blood cells) has been detected. No prior in vitro incubation of freshly drawn and isolated peripheral blood lymphocytes is required for the expression of the IgM antibody binding receptor on T cells. Results obtained in experiments designed for the simultaneous detection of lymphocytes reacting with specific rhodamine labelled antihuman-T cell antiserum and forming rosettes with EA-IgM complexes of Esh and the IgM antiserum fraction showed the receptor for IgM antibody to be present exclusively on T cells. Pre-incubation of T cells with free IgM or its Fc fragments leads to inhibition of rosette formation with EA-IgM. Treatment of the IgM type pentameric antibody with dithioerythritol to cleave pentameric IgM reduces the percentage of EA-IgM rosette forming cells (RFC) significantly. The possible biological significance of this receptor is discussed.
已检测到T淋巴细胞上一种能结合IgM型抗体的受体，该受体在抗体与Esh（绵羊红细胞）预先形成复合物之前以及之后均可结合。T细胞上IgM抗体结合受体的表达无需对新抽取并分离的外周血淋巴细胞进行体外预孵育。在旨在同时检测与特定罗丹明标记的抗人T细胞抗血清反应并与Esh的EA-IgM复合物和IgM抗血清组分形成玫瑰花结的淋巴细胞的实验中获得的结果表明，IgM抗体受体仅存在于T细胞上。用游离IgM或其Fc片段对T细胞进行预孵育会导致与EA-IgM形成玫瑰花结的过程受到抑制。用二硫苏糖醇处理IgM型五聚体抗体以裂解五聚体IgM，可显著降低EA-IgM玫瑰花结形成细胞（RFC）的百分比。本文讨论了该受体可能的生物学意义。
