Khan Inam Ullah, Habib Neeli, Xiao Min, Li Meng-Meng, Xian Wen-Dong, Hejazi Mohammad Saeid, Tarhriz Vahideh, Zhi Xiao-Yang, Li Wen-Jun
Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People's Republic of China.
Department of Biological Sciences, Gomal University, Dera Ismail Khan, KPK, Pakistan.
Antonie Van Leeuwenhoek. 2019 Jun;112(6):867-875. doi: 10.1007/s10482-018-01219-7. Epub 2019 Feb 23.
An ovoid to rod-shaped, phototrophic, purple non-sulfur bacterium was isolated from a sediment sample of a hot spring in Tibet, China. Cells of strain YIM 73036 were Gram-stain negative, non-motile and multiplied by binary fission. Strain YIM 73036 grew optimally at pH 7.0-7.5 at 37-45 °C. Growth occurred in 0.5-3.5% (w/v) NaCl. Vitamins were not required for growth. The presence of photosynthesis genes pufL and pufM were shown and photosynthesis pigments were formed. Bacteriochlorophyll α, the bacteriopheophytin and carotenoids were present as photosynthetic pigments. Internal cytoplasmic membranes were of the lamellar type. The organism YIM 73036 was able to grow chemo-organoheterophically, chemo-lithoautotrophically and photo-organoheterotrophically but photo-lithoautotrophic and fermentative growth were not demonstrated. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain YIM 73036 is closely related to Rhodobacter blasticus ATCC 33485 (96.65% sequence similarity) and clustered with species of the genus Rhodobacter of the family Rhodobacteraceae. Whole-genome sequence analyses based on the average nucleotide BLAST identity (ANI < 82%) indicated that this isolate belongs to a novel species. The genomic DNA G+C content of organism YIM 73036 was determined to be 66.0 mol%. Strain YIM 73036 contained Q-10 as the predominant ubiquinone and Cω7c, Cω7c 11-methyl and C as the major fatty acids. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and unidentified phospholipid. Differential phenotypic and chemotaxonomic properties, together with the phylogenetic distinctiveness, demonstrated that strain YIM 73036 is distinguishable from other species of the genus Rhodobacter. On the basis of the data presented, strain YIM 73036 is considered to represent a novel species of the genus Rhodobacter, for which the name Rhodobacter thermarum sp. nov. [type strain YIM 73036 (= KCTC 52712 = CCTCC AB 2016298)] is proposed.
从中国西藏一处温泉的沉积物样本中分离出一种卵形至杆状、光合型的紫色非硫细菌。YIM 73036菌株的细胞革兰氏染色呈阴性，无运动性，通过二分裂进行繁殖。YIM 73036菌株在37 - 45℃、pH 7.0 - 7.5条件下生长最佳。在0.5 - 3.5%（w/v）NaCl中可生长。生长不需要维生素。检测到光合作用基因pufL和pufM的存在，并形成了光合作用色素。光合色素包括细菌叶绿素α、细菌脱镁叶绿素和类胡萝卜素。内部细胞质膜为层状类型。YIM 73036菌株能够进行化能有机异养、化能无机自养和光合有机异养生长，但未证明其能进行光合无机自养和发酵生长。基于16S rRNA基因序列的系统发育分析表明，YIM 73036菌株与 Blastobacter blasticus ATCC 33485密切相关（序列相似性为96.65%），并与红杆菌科红杆菌属的物种聚类。基于平均核苷酸BLAST同一性（ANI < 82%）的全基因组序列分析表明，该分离株属于一个新物种。YIM 73036菌株的基因组DNA G + C含量测定为66.0 mol%。YIM 73036菌株以Q - 10作为主要泛醌，以Cω7c、Cω7c 11 - 甲基和C作为主要脂肪酸。主要极性脂为磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和未鉴定的磷脂。不同的表型和化学分类学特性，以及系统发育独特性，表明YIM 73036菌株与红杆菌属的其他物种不同。根据所提供的数据，YIM 73036菌株被认为代表红杆菌属的一个新物种，为此提出名称为热红杆菌（Rhodobacter thermarum sp. nov.）[模式菌株YIM 73036（= KCTC 52712 = CCTCC AB 2016298）]。
