Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.
Genes (Basel). 2023 Jul 29;14(8):1556. doi: 10.3390/genes14081556.
Salt stress is an important environmental factor affecting crop growth and development. One of the important ways to improve the salt tolerance of rice is to identify new salt-tolerance genes, reveal possible mechanisms, and apply them to the creation of new germplasm and the breeding of new varieties. In this study, the salt-sensitive japonica variety Tong 35 (T35) and salt-tolerant japonica variety Ji Nongda 709 (JND709) were used. Salt stress treatment with a 150 mmol/L NaCl solution (the control group was tested without salt stress treatment simultaneously) was continued until the test material was collected after the rice germination period. Twelve cDNA libraries were constructed, and 5 comparator groups were established for transcriptome sequencing. On average, 9.57G of raw sequencing data were generated per sample, with alignment to the reference genome above 96.88% and alignment to guanine-cytosine (GC) content above 53.86%. A total of 16,829 differentially expressed genes were present in the five comparison groups, of which 2390 genes were specifically expressed in T35 (category 1), 3306 genes were specifically expressed in JND709 (category 2), and 1708 genes were differentially expressed in both breeds (category 3). Differentially expressed genes were subjected to gene ontology (GO), functional enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, which revealed that these genes belonged to three main classes: molecular function, cellular components, and biological processes. KEGG pathway analysis showed that the significantly enriched pathways for these differentially expressed genes included phenylpropane biosynthesis, phytohormone signaling, and the interaction of plants with pathogens. In this study, we provided a reference for studying the molecular mechanism underlying salt tolerance during germination.
盐胁迫是影响作物生长和发育的重要环境因素之一。提高水稻耐盐性的重要途径之一是鉴定新的耐盐基因，揭示可能的机制，并将其应用于新种质的创造和新品种的选育。本研究以盐敏感粳稻品种通 35（T35）和盐耐受粳稻品种吉农大 709（JND709）为材料，用 150mmol/L NaCl 溶液进行盐胁迫处理（同时设置无盐胁迫处理的对照组），直至发芽期结束后采集试验材料。构建了 12 个 cDNA 文库，建立了 5 个比较组进行转录组测序。每个样本平均产生 9.57G 的原始测序数据，比对参考基因组的比例高于 96.88%，比对鸟嘌呤-胞嘧啶（GC）含量的比例高于 53.86%。在五个比较组中共检测到 16829 个差异表达基因，其中 T35 特异表达的基因有 2390 个（类别 1），JND709 特异表达的基因有 3306 个（类别 2），两个品种共表达的差异表达基因有 1708 个（类别 3）。对差异表达基因进行基因本体（GO）、功能富集分析和京都基因与基因组百科全书（KEGG）通路分析，结果表明这些基因主要属于三大类：分子功能、细胞成分和生物过程。KEGG 通路分析表明，这些差异表达基因显著富集的通路包括苯丙烷生物合成、植物激素信号转导和植物与病原体的相互作用。本研究为研究发芽期耐盐性的分子机制提供了参考。
