Academic Veterinary Medicine Unit, Universidade Federal de Campina Grande, Patos, Paraíba, Brazil.
Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
J Glob Antimicrob Resist. 2018 Dec;15:252-253. doi: 10.1016/j.jgar.2018.10.023. Epub 2018 Nov 4.
Escherichia coli strains producing extended-spectrum β-lactamases (ESBLs), especially CTX-M-type, have been largely described in companion animals; however, genomic data are lacking to clarify the clinical impact of ESBL-producing isolates in these hosts. The aim of this study was to present the genomic features of a highly virulent, ceftiofur-resistant, CTX-M-8-producing E. coli isolate from a case of pneumonia in a domestic cat with fatal outcome.
Genomic DNA was sequenced using an Illumina NextSeq 500 platform and was assembled using CLC Genomic Workbench. Genomic data were analysed using online bioinformatics tools.
The genome size was evaluated at 5.1Mb, with 5334 protein-coding sequences. The strain was assigned to sequence type 224 (ST224) and presented genes conferring resistance to β-lactams (bla), sulphonamides (sul2), tetracycline (tetA) and trimethoprim (dfrA14) as well as chromosomal point mutations in ParC (S80I), GyrA (S83L) and GyrB (D87N). In addition, the presence of the virulence genes cba, gad, ipfA, iroN, iss, mchF and tsh was detected.
This draft genome sequence might provide important data for a better understanding of genomic aspects regarding the dissemination of CTX-M-8-producing E. coli in the human-animal-environment interface.
产生超广谱β-内酰胺酶（ESBLs）的大肠杆菌菌株，特别是 CTX-M 型，在伴侣动物中已被大量描述；然而，缺乏基因组数据来阐明这些宿主中产生 ESBL 的分离株的临床影响。本研究旨在介绍一例肺炎患猫中分离出的高毒力、头孢噻呋耐药、CTX-M-8 产生的大肠杆菌的基因组特征，该猫最终死亡。
使用 Illumina NextSeq 500 平台对基因组 DNA 进行测序，并使用 CLC Genomic Workbench 进行组装。使用在线生物信息学工具分析基因组数据。
基因组大小评估为 5.1Mb，包含 5334 个蛋白质编码序列。该菌株被分配到序列型 224（ST224），并呈现出对β-内酰胺类（bla）、磺胺类（sul2）、四环素（tetA）和甲氧苄啶（dfrA14）的耐药基因，以及 ParC（S80I）、GyrA（S83L）和 GyrB（D87N）的染色体点突变。此外，还检测到毒力基因 cba、gad、ipfA、iroN、iss、mchF 和 tsh 的存在。
该草图基因组序列可能为更好地理解 CTX-M-8 产生的大肠杆菌在人类-动物-环境界面传播的基因组方面提供重要数据。
