Department of Morphology, Federal University of Sergipe, São Cristóvão, Brazil; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
J Glob Antimicrob Resist. 2018 Dec;15:288-289. doi: 10.1016/j.jgar.2018.10.024. Epub 2018 Nov 4.
Genomic surveillance studies monitoring the dissemination of multidrug-resistant Enterobacteriaceae in polluted aquatic ecosystems are urgently required. The aim of this study was to report the draft genome sequence of an MCR-1 and CTX-M-8 co-producing Escherichia coli isolated from a polluted mangrove ecosystem in Northeast Brazil.
Total genomic DNA was sequenced on an Illumina NextSeq platform and was assembled using CLC Genomics Workbench. The whole-genome sequence was evaluated through bioinformatics tools available from the Center of Genomic Epidemiology as well as additional in silico analysis.
The genome size was calculated at 5089467bp, comprising a total of 5068 protein-coding sequences. The strain was assigned to sequence type 58 (ST58) and revealed the presence of mcr-1, bla and other clinically significant genes responsible for conferring resistance to colistin, β-lactams, trimethoprim and quinolones. In addition, genes conferring resistance to silver (silR) and quaternary ammonium compounds (sugE) were identified.
These data provide valuable information for comparative genomic analysis regarding the dissemination of MCR-1-producing E. coli at the human-animal-environment interface.
迫切需要开展监测多药耐药肠杆菌科在受污染水生生态系统中传播的基因组监测研究。本研究旨在报告从巴西东北部受污染红树林生态系统中分离出的一株同时携带 MCR-1 和 CTX-M-8 的产 MCR-1 和 CTX-M-8 大肠杆菌的基因组序列草案。
使用 Illumina NextSeq 平台对总基因组 DNA 进行测序，并使用 CLC Genomics Workbench 进行组装。通过基因组流行病学中心提供的生物信息学工具以及其他计算机分析对全基因组序列进行评估。
基因组大小计算为 5089467bp，共包含 5068 个蛋白质编码序列。该菌株被分配到序列型 58（ST58），并显示出 mcr-1、bla 和其他其他临床重要基因的存在，这些基因负责赋予对粘菌素、β-内酰胺类、甲氧苄啶和喹诺酮类的耐药性。此外，还鉴定出了赋予对银（silR）和季铵化合物（sugE）耐药性的基因。
这些数据为人类-动物-环境界面中 MCR-1 产生的大肠杆菌传播的比较基因组分析提供了有价值的信息。
