Afrasiabi Roodabeh, Soderberg Lovisa M, Joensson Haakan N, Björk Per, Andersson Svahn Helene, Linnros Jan
Materials and Nano Physics, School of Information and Communication Technology, KTH Royal Institute of Technology, SE-164 40 Kista, Sweden.
Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of Technology, SE-171 65 Solna, Sweden.
Micromachines (Basel). 2016 Aug 5;7(8):134. doi: 10.3390/mi7080134.
We present a novel microfluidic system that integrates droplet microfluidics with a silicon nanoribbon field-effect transistor (SiNR FET), and utilize this integrated system to sense differences in pH. The device allows for selective droplet transfer to a continuous water phase, actuated by dielectrophoresis, and subsequent detection of the pH level in the retrieved droplets by SiNR FETs on an electrical sensor chip. The integrated microfluidic system demonstrates a label-free detection method for droplet microfluidics, presenting an alternative to optical fluorescence detection. In this work, we were able to differentiate between droplet trains of one pH-unit difference. The pH-based detection method in our integrated system has the potential to be utilized in the detection of biochemical reactions that induce a pH-shift in the droplets.
我们展示了一种新型微流控系统，该系统将液滴微流控与硅纳米带场效应晶体管（SiNR FET）集成在一起，并利用这个集成系统来检测pH值的差异。该装置允许通过介电泳将选择性液滴转移到连续水相中，并随后通过电传感器芯片上的SiNR FET检测回收液滴中的pH值水平。该集成微流控系统展示了一种用于液滴微流控的无标记检测方法，为光学荧光检测提供了一种替代方案。在这项工作中，我们能够区分pH值相差一个单位的液滴序列。我们集成系统中基于pH值的检测方法有潜力用于检测在液滴中引起pH值变化的生化反应。
