Belyaev Dmitry, Schütt Julian, Ibarlucea Bergoi, Rim Taiuk, Baraban Larysa, Cuniberti Gianaurelio
Max Bergmann Center of Biomaterials and Institute for Materials Science, Technische Universität Dresden, 01069 Dresden, Germany.
Technische Universität Dresden, Center for Advancing Electronics Dresden, 01062 Dresden, Germany.
Micromachines (Basel). 2020 Jan 26;11(2):138. doi: 10.3390/mi11020138.
Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of β-galactosidase can be one of the markers for several diseases, ., cancer, hyper/hypoglycemia, cell senescence, or other disruptions in cell functioning. We measure the reaction and reaction kinetics-associated shift of the source-to-drain current in the system, which is caused by the change of the ionic strength of the microenvironment. With these results, we demonstrate that the ion-sensitive FETs are able to sense the interior of the aqueous reactors; thus, the conjunction of miniature nanosensors and droplet-based microfluidic systems conceptually opens a new route toward a sensitive, optics-less analysis of biochemical processes.
在此，我们展示了一种用于对包含基于纳米硅纳米线的场效应晶体管（FET）的乳液液滴进行全电动、无标记分析的微型芯片实验室检测系统。我们特别关注β - 半乳糖苷酶活性的分析，β - 半乳糖苷酶是糖酵解代谢途径中的一种重要酶。此外，β - 半乳糖苷酶的合成和作用效率可能是多种疾病的标志物之一，例如癌症、高血糖/低血糖、细胞衰老或细胞功能的其他紊乱。我们测量系统中源极 - 漏极电流与反应及反应动力学相关的变化，这是由微环境离子强度的变化引起的。基于这些结果，我们证明离子敏感型FET能够感知水相反应器的内部；因此，微型纳米传感器与基于液滴的微流体系统的结合从概念上为生物化学过程灵敏、无光学分析开辟了一条新途径。
