利用微流控芯片快速捕获和分析医院空气中的金黄色葡萄球菌。
Rapid Capture and Analysis of Airborne Staphylococcus aureus in the Hospital Using a Microfluidic Chip.
作者信息
Jiang Xiran, Liu Yingchao, Liu Qi, Jing Wenwen, Qin Kairong, Sui Guodong
机构信息
Department of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China.
Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
出版信息
Micromachines (Basel). 2016 Sep 15;7(9):169. doi: 10.3390/mi7090169.
In this study we developed a microfluidic chip for the rapid capture, enrichment and detection of airborne () . The whole analysis took about 4 h and 40 min from airborne sample collection to loop-mediated isothermal amplification (LAMP), with a detection limit down to about 27 cells. The process did not require DNA purification. The chip was validated using standard bacteria bioaerosol and was directly used for clinical airborne pathogen sampling in hospital settings. This is the first report on the capture and analysis of airborne using a novel microfluidic technique, a process that could have a very promising platform for hospital airborne infection prevention (HAIP).
在本研究中，我们开发了一种微流控芯片，用于快速捕获、富集和检测空气中的（）。从采集空气样本到环介导等温扩增（LAMP），整个分析过程耗时约4小时40分钟，检测限低至约27个细胞。该过程无需DNA纯化。该芯片使用标准细菌生物气溶胶进行了验证，并直接用于医院环境中的临床空气传播病原体采样。这是关于使用新型微流控技术捕获和分析空气中（）的首次报告，该过程可能为医院空气传播感染预防（HAIP）提供一个非常有前景的平台。
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