State Key Laboratory of Food Science and Technology School of Food Science National Engineering Research Center for Functional Foods, Synergetic Innovation Center of Food Safety, Joint International Research Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China.
Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbiology Culture Collection and Application Guangdong Open Laboratory of Applied Microbiology, Guangzhou, China.
Crit Rev Food Sci Nutr. 2020;60(2):201-224. doi: 10.1080/10408398.2018.1518897. Epub 2018 Dec 20.
Due to the significant growth of food production, the potential likelihood of food contamination is increasing. Foodborne illness caused by bacterial pathogens has considerably increased over the past decades, while at the same time, the species of harmful microorganisms also varied. Conventional bacterial culturing methods have been unable to satisfy the growing requirement for food safety inspections and food quality assurance. Therefore, rapid and simple detection methods are urgently needed. The loop-mediated isothermal amplification (LAMP) technology is a highly promising approach for the rapid and sensitive detection of pathogens, which allows nucleic acid amplification under isothermal conditions. The integration of the LAMP assay onto a microfluidic chip is highly compatible with point-of-care or resource-limited settings, as it offers the capability to perform experiments in combination with high screening efficiency. Here, we provide an overview of recent advances in LAMP-based microfluidic chip technology for detecting pathogens, based on real-time or endpoint determination mechanisms. We also discuss the promoting aspects of using the LAMP technique in a microfluidic platform, to supply a guideline for further molecular diagnosis and genetic analysis.
由于食品生产的显著增长，食品污染的可能性也在增加。在过去几十年中，由细菌病原体引起的食源性疾病大大增加，同时，有害微生物的种类也有所不同。传统的细菌培养方法已经不能满足日益增长的食品安全检查和食品质量保证的要求。因此，迫切需要快速简单的检测方法。环介导等温扩增（LAMP）技术是一种很有前途的病原体快速灵敏检测方法，它可以在等温条件下进行核酸扩增。将 LAMP 分析与微流控芯片集成非常适合于即时或资源有限的环境，因为它能够与高通量筛选相结合进行实验。在这里，我们根据实时或终点测定机制，综述了基于 LAMP 的微流控芯片技术在检测病原体方面的最新进展。我们还讨论了在微流控平台中使用 LAMP 技术的促进方面，为进一步的分子诊断和遗传分析提供了指导。
