一种使用聚二甲基硅氧烷印章制造微结构的简单方法。
A Simple Method for Fabrication of Microstructures Using a PDMS Stamp.
作者信息
Lee Hun, Koh Domin, Xu Linfeng, Row Sindhu, Andreadis Stelios T, Oh Kwang W
机构信息
Sensors and MicroActuators Learning Laboratory (SMALL), Department of Electrical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA.
Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA.
出版信息
Micromachines (Basel). 2016 Oct 1;7(10):173. doi: 10.3390/mi7100173.
We report a simple method to fabricate PDMS (polydimethylsiloxane) microwell arrays on glass by using a PDMS stamp to study cell-to-cell adhesion. In the cell-to-cell study, a glass substrate is required since glass has better cell attachment. The microwell arrays are replicated from an SU-8 master mold, and then are transferred to a glass substrate by lifting the PDMS stamp, followed by oxygen plasma bonding of the PDMS stamp on the glass substrate. For the cell-to-cell adhesion, four different types of PDMS arrays (e.g., rectangle, bowtie, wide-rhombus, and rhombus) were designed to vary the cell-to-cell contact length. The transfer success rates of the microwell arrays were measured as a function of both the contact area of the PDMS and the glass substrate and the different ratios between the base polymers and the curing agent. This method of generating the microwell arrays will enable a simple and robust construction of PDMS-based devices for various biological applications.
我们报告了一种通过使用聚二甲基硅氧烷（PDMS）印章在玻璃上制造PDMS微孔阵列的简单方法，以研究细胞间粘附。在细胞间研究中，由于玻璃具有更好的细胞附着性，因此需要使用玻璃基板。微孔阵列从SU-8主模具复制而来，然后通过抬起PDMS印章转移到玻璃基板上，随后将PDMS印章氧等离子体键合到玻璃基板上。为了研究细胞间粘附，设计了四种不同类型的PDMS阵列（例如矩形、领结形、宽菱形和菱形）以改变细胞间接触长度。测量了微孔阵列的转移成功率与PDMS和玻璃基板的接触面积以及基础聚合物与固化剂之间不同比例的函数关系。这种生成微孔阵列的方法将能够简单而稳健地构建用于各种生物应用的基于PDMS的设备。
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