通过紫外纳米压印光刻技术制造的用于自动神经元细胞分析读出的微图案微孔板的新概念。

Lohse Mirko, Thesen Manuel W, Haase Anja, Smolka Martin, Iceta Nerea Briz, Ayerdi Izquierdo Ana, Ramos Isbaal, Salado Clarisa, Schleunitz Arne

Micro Resist Technology GmbH, Köpenicker Str. 325, 12555 Berlin, Germany.

Joanneum Research Materials, Institute for Surface Technologies and Photonics, 8160 Weiz, Austria.

Nanomaterials (Basel). 2021 Apr 1;11(4):902. doi: 10.3390/nano11040902.

The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.

描述了通过紫外纳米压印光刻（UV-NIL）制造新型微米级通道网络，形成开放通道微流控系统的过程。介绍了从母版制作到小批量制造以及每小时高达1200个微量滴定板的高通量制造的完整工艺流程细节。深入探讨了对一种合适的紫外光固化材料mr-UVCur26SF的评估，包括细胞毒性评估、细胞相容性测试以及最终的神经元分析。结果表明，给定的图案与抗蚀剂相结合，为更快、更便宜和更可靠的药物筛选铺平了道路。