Kim Sung Eun, Kim Min Sung, Shin Yong Cheol, Eom Seong Un, Lee Jong Ho, Shin Dong-Myeong, Hong Suck Won, Kim Bongju, Park Jong-Chul, Shin Bo Sung, Lim Dohyung, Han Dong-Wook
Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Korea.
Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Korea.
Micromachines (Basel). 2016 Oct 14;7(10):186. doi: 10.3390/mi7100186.
Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds.
光刻是一种独特的工艺，能够在各种衬底上有效地制造微/纳米尺寸的图案。另一方面，弯月面拖动沉积（MDD）工艺可以使衬底表面均匀。氧化石墨烯（GO）是石墨烯的氧化形式，具有高亲水性和蛋白质吸附性。它广泛应用于生物医学领域，如药物递送、再生医学和组织工程。在此，我们通过MDD和光刻制造了均匀的GO微图案。通过原子力显微镜（AFM）、扫描电子显微镜（SEM）和拉曼光谱对GO微图案的物理化学性质进行了表征。此外，研究了细胞在GO微图案上的迁移情况，并考察了三角形和方形GO微图案上细胞迁移的差异对细胞迁移的影响。我们的结果表明，通过MDD和光刻可以精细地制造出具有所需形状的GO微图案。此外，研究还发现GO微图案的形状在细胞迁移距离、速度和方向性方面起着关键作用。因此，我们的研究结果表明，GO微图案可作为一种有前途的生物功能平台和细胞导向衬底，应用于生物电子器件、芯片上细胞和组织工程支架。
