Yoon Kyung Jae, Kim Dae Yul
Department of Physical and Rehabilitation Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University, School of Medicine, Seoul, Korea.
Medical Research Institute, Regenerative and Neuroscience Laboratory, Kangbuk Samsung Hospital, Sungkyunkwan University, School of Medicine, Seoul, Korea.
Ann Rehabil Med. 2018 Oct;42(5):643-651. doi: 10.5535/arm.2018.42.5.643. Epub 2018 Oct 31.
To evaluate the immediate effect of single exercise on physical performance and memory in the early stage of traumatic brain injury (TBI) in rats.
Ninety TBI rats were randomly assigned to T0 (sedentary), T10 (treadmill 10 m/min for 30 minutes), or T20 (treadmill 20 m/min for 30 minutes) groups, on day 3 (D3), D7, and D14 after TBI, respectively. Rotarod (RR), Barnes maze (BM), brain magnetic resonance imaging (MRI) and MR spectroscopy were performed immediately before and 6 hours after exercise. Rats were sacrificed for immunohistochemistry with heat shock protein 70 (Hsp70) and glial fibrillary acidic protein (GFAP).
On D3, the T10 and T20 groups demonstrated significant improvement in RR (p<0.05). On D7, only the T20 group showed significantly enhanced RR (p<0.05). In BM on D3, the T20 group showed significant deterioration compared with the other groups (p<0.05). Lesion volume did not significantly differ among the groups. MR spectroscopy on D3 showed that only the T20 group had significantly increased choline/creatine and 0.9/creatine (p<0.05). In the perilesional area on D3, only T20 had a significantly higher Hsp70 and GFAP than the T0 group. On D7, Hsp70 was significantly higher in the T20 group than in the T0 group (p<0.05). In the ipsilesional hippocampus on D3, the T20 group showed a significantly higher Hsp70 and GFAP than the T0 group (p<0.05).
A single session of low-intensity exercise in the early period of TBI improves behavioral performance without inducing cognitive deficits. However, high-intensity exercise can exacerbate cognitive function in the early period after TBI. Therefore, the optimal timing of rehabilitation and exercise intensity are crucial in behavior and memory recovery after TBI.
评估单次运动对大鼠创伤性脑损伤（TBI）早期身体机能和记忆的即时影响。
90只TBI大鼠分别在TBI后的第3天（D3）、第7天和第14天随机分为T0（久坐不动）、T10（跑步机速度10米/分钟，持续30分钟）或T20（跑步机速度20米/分钟，持续30分钟）组。在运动前及运动后6小时立即进行转棒试验（RR）、巴恩斯迷宫试验（BM）、脑磁共振成像（MRI）和磁共振波谱分析。处死大鼠进行热休克蛋白70（Hsp70）和胶质纤维酸性蛋白（GFAP）的免疫组织化学检测。
在D3时，T10和T20组的RR表现出显著改善（p<0.05）。在D7时，只有T20组的RR显著增强（p<0.05）。在D3的BM试验中，T20组与其他组相比表现出显著恶化（p<0.05）。各组间损伤体积无显著差异。D3的磁共振波谱分析显示，只有T20组的胆碱/肌酸和0.9/肌酸显著增加（p<0.05）。在D3的损伤周边区域，只有T20组的Hsp70和GFAP显著高于T0组。在D7时，T20组的Hsp70显著高于T0组（p<0.05）。在D3的同侧海马区，T20组的Hsp70和GFAP显著高于T0组（p<0.05）。
TBI早期进行单次低强度运动可改善行为表现而不诱发认知缺陷。然而，高强度运动可在TBI后的早期加重认知功能。因此，康复的最佳时机和运动强度对于TBI后的行为和记忆恢复至关重要。
