Taylor-Papadimitriou J, Shearer M, Stoker M G
Int J Cancer. 1977 Dec 15;20(6):903-8. doi: 10.1002/ijc.2910200613.
Colony-forming epithelial cells can be separated from the non-dividing "foam cells" in human milk by differential adhesion to glass and freezing. The growth of such partially purified mammary epithelial cells is stimulated by co-culture with non-dividing feeder cells. Foam cells, mitomycin-treated mouse fibroblast lines and human mammary fibroblasts and calf lens epithelial cells are all effective in promoting mammary epithelial cell growth. Contact between epithelial cells and feeders is not required for the growth-promoting effect. The mitogenic effect of epidermal growth factor on mammary epithelial cells also requires feeder cell activity.
通过对玻璃的差异黏附及冷冻处理，可从人乳中的非分裂“泡沫细胞”中分离出形成集落的上皮细胞。这种部分纯化的乳腺上皮细胞与非分裂饲养细胞共培养时，其生长会受到刺激。泡沫细胞、经丝裂霉素处理的小鼠成纤维细胞系、人乳腺成纤维细胞以及小牛晶状体上皮细胞在促进乳腺上皮细胞生长方面均有效。上皮细胞与饲养细胞之间的接触并非生长促进效应所必需。表皮生长因子对乳腺上皮细胞的促有丝分裂作用也需要饲养细胞的活性。
