Fukaya Y, Hara T, Hara T, Iwata S
Department of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Meijo University, Nagoya, Japan.
J Cataract Refract Surg. 1988 May;14(3):309-11. doi: 10.1016/s0886-3350(88)80122-6.
The effect of freezing on the growth of rat lens epithelial cells was studied in vitro. We found that 80% of the lens epithelial cells died after freezing at -45 degrees C for two hours and that the surviving cells could grow with the addition of growth factors or when placed on a sheet of type 4 collagen, but not when placed on a plain plastic culture dish. These results suggest that the surviving cells are at the Go phase of the cell cycle and that type 4 collagen or growth factors can initiate cell division.
体外研究了冷冻对大鼠晶状体上皮细胞生长的影响。我们发现，在-45℃冷冻两小时后，80%的晶状体上皮细胞死亡，存活的细胞在添加生长因子或置于IV型胶原片上时能够生长，但置于普通塑料培养皿上时则不能生长。这些结果表明，存活的细胞处于细胞周期的G0期，IV型胶原或生长因子可启动细胞分裂。
