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橙皮苷通过抑制细胞迁移和侵袭、使细胞周期停滞以及诱导线粒体介导的凋亡，在人骨肉瘤MG-63细胞和异种移植小鼠模型中表现出抗肿瘤作用。

Hesperidin exhibits and antitumor effects in human osteosarcoma MG-63 cells and xenograft mice models via inhibition of cell migration and invasion, cell cycle arrest and induction of mitochondrial-mediated apoptosis.

作者信息

Du Guang-Yu, He Sheng-Wei, Zhang Lu, Sun Chuan-Xiu, Mi Li-Dong, Sun Zue-Gang

机构信息

Department of Bone Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.

出版信息

Oncol Lett. 2018 Nov;16(5):6299-6306. doi: 10.3892/ol.2018.9439. Epub 2018 Sep 14.

DOI:10.3892/ol.2018.9439
PMID:30405765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6202547/
Abstract

The objective of the present study was to investigate the anticancer properties of hesperidin against human osteosarcoma MG-63 cells. Its effects on apoptosis, cell migration, cell invasion and cell cycle arrest, and its effects on tumor volume and weight were also evaluated in the present study. MTS assay was used to study the cytotoxic effects of the compound on cell viability. Effects on apoptosis and cell cycle arrest were evaluated by flow cytometry. wound healing assay and Matrigel assay were performed to study the effects of hesperidin on cell migration and cell invasion, respectively. Hesperidin exerted dose-dependent and time-dependent growth inhibitory effects on cervical cancer cells with IC50 values of 33.5, 23.8 and 17.6 µM, respectively, at 24, 48 and 72 h time intervals. Hesperidin led to early and late apoptosis induction in these cells. Hesperidin-treated cells also led to G2/M phase cell cycle arrest, which exhibited strong dose-dependence. Hesperidin treatment also led to inhibition of cell migration and invasion.

摘要

本研究的目的是探究橙皮苷对人骨肉瘤MG-63细胞的抗癌特性。本研究还评估了其对细胞凋亡、细胞迁移、细胞侵袭和细胞周期阻滞的影响，以及对肿瘤体积和重量的影响。采用MTS法研究该化合物对细胞活力的细胞毒性作用。通过流式细胞术评估对细胞凋亡和细胞周期阻滞的影响。分别进行划痕实验和基质胶实验以研究橙皮苷对细胞迁移和细胞侵袭的影响。橙皮苷对宫颈癌细胞具有剂量依赖性和时间依赖性的生长抑制作用，在24、48和72小时间隔时的IC50值分别为33.5、23.8和17.6μM。橙皮苷导致这些细胞发生早期和晚期凋亡。经橙皮苷处理的细胞还导致G2/M期细胞周期阻滞，表现出很强的剂量依赖性。橙皮苷处理还导致细胞迁移和侵袭受到抑制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/e17fd00b6763/ol-16-05-6299-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/4b07875c5ca5/ol-16-05-6299-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/ca0271a3321d/ol-16-05-6299-g01.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/c9cab5d7d5c0/ol-16-05-6299-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/6ae3631fbb83/ol-16-05-6299-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/8568b91502f8/ol-16-05-6299-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/c38fbf7a329f/ol-16-05-6299-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/e17fd00b6763/ol-16-05-6299-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/4b07875c5ca5/ol-16-05-6299-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/ca0271a3321d/ol-16-05-6299-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/07d5adbf0175/ol-16-05-6299-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/5aa8042d9ca4/ol-16-05-6299-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/c9cab5d7d5c0/ol-16-05-6299-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/6ae3631fbb83/ol-16-05-6299-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/8568b91502f8/ol-16-05-6299-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/c38fbf7a329f/ol-16-05-6299-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e86/6202547/e17fd00b6763/ol-16-05-6299-g08.jpg

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