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B族链球菌β-溶血素生物合成的转座子诱变

Transposon mutagenesis of group B streptococcus beta-hemolysin biosynthesis.

作者信息

Weiser J N, Rubens C E

出版信息

Infect Immun. 1987 Sep;55(9):2314-6. doi: 10.1128/iai.55.9.2314-2316.1987.

DOI:10.1128/iai.55.9.2314-2316.1987
PMID:3040595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260700/
Abstract

Beta-hemolysin production by group B streptococci (GBS) is speculated to be a major virulence factor of the organism. A virulent, beta-hemolytic group B streptococcus strain was mutagenized with the self-conjugative transposon Tn916 to derive isogenic strains with mutations only in the gene(s) responsible for beta-hemolysin biosynthesis. There was no significant difference between the virulence of the parent strain and that of the mutant strains in a neonatal rat sepsis model.

摘要

B族链球菌（GBS）产生的β-溶血素被推测为该生物体的主要毒力因子。用自我接合转座子Tn916对一株有毒的β-溶血B族链球菌菌株进行诱变，以获得仅在负责β-溶血素生物合成的基因中发生突变的同基因菌株。在新生大鼠败血症模型中，亲本菌株和突变菌株的毒力没有显著差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d206/260700/2cd2f8cf53d4/iai00093-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d206/260700/2cd2f8cf53d4/iai00093-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d206/260700/2cd2f8cf53d4/iai00093-0375-a.jpg

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