Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
Front Cell Infect Microbiol. 2018 Oct 18;8:366. doi: 10.3389/fcimb.2018.00366. eCollection 2018.
After invasion, resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane. This suggests that secretion of GRA beyond the PVM is not important for the tissue cyst stage of .
入侵后， 虫体寄生于一个被 PV 膜（PVM）包围的寄生空泡（PV）中。一旦进入 PV，速殖子就会分泌致密颗粒蛋白（GRAs），其中一些，如 GRA16 和 GRA24，可能通过一个假定的转位器运送到 PVM 之外。然而，一旦速殖子在包囊内转化为缓殖子，尚不清楚分泌的 GRAs 是否能够穿过囊壁膜。我们使用四环素诱导系统在诱导阶段转换后驱动 HA 表位标记的 GRA16 和 GRA24 的表达，并表明这些蛋白不会分泌到囊壁膜之外。这表明，GRA 穿过 PVM 的分泌对于 的组织包囊阶段并不重要。
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