Park Doori, Park Su-Hyun, Kim Youn Shic, Choi Beom-Soon, Kim Ju-Kon, Kim Nam-Soo, Choi Ik-Young
Department of Molecular Bioscience, Kangwon National University, Chuncheon, South Korea.
Graduate School of International Agricultural Technology and Crop Biotech Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, South Korea.
Genes Genomics. 2019 Feb;41(2):213-222. doi: 10.1007/s13258-018-0754-5. Epub 2018 Nov 7.
As the transformation process can induce mutations in host plants, molecular characterization of the associated genomic changes is important not only for practical food safety but also for understanding the fundamental theories of genome evolution.
To investigate a population-scale comparative study of the genome-wide spectrum of sequence variants in the transgenic genome with the variations present in 3000 rice varieties.
On average, we identified 19,273 SNPs (including Indels) per transgenic line in which 10,729 SNPs were at the identical locations in the three transgenic rice plants. We found that these variations were predominantly present in specific regions in chromosomes 8 and 10. Majority (88%) of the identified variations were detected at the same genomic locations as those in natural rice population, implying that the transgenic induced mutations had a tendency to be common alleles.
Genomic variations in transgenic rice plants frequently occurred at the same sites as the major alleles found in the natural rice population, which implies that the sequence variations occur within the limits of a biological system to ensure survival.
由于转化过程可诱导宿主植物发生突变，因此对相关基因组变化进行分子特征分析不仅对实际食品安全很重要，而且对于理解基因组进化的基本理论也很重要。
开展一项群体规模的比较研究，分析转基因基因组中序列变异的全基因组谱与3000个水稻品种中存在的变异情况。
平均而言，我们在每个转基因株系中鉴定出19273个单核苷酸多态性（包括插入缺失），其中10729个单核苷酸多态性在三株转基因水稻植株的相同位置。我们发现这些变异主要存在于第8号和第10号染色体的特定区域。所鉴定变异的大多数（88%）在与天然水稻群体相同的基因组位置被检测到，这意味着转基因诱导的突变倾向于成为常见等位基因。
转基因水稻植株中的基因组变异经常发生在与天然水稻群体中主要等位基因相同的位点，这意味着序列变异发生在生物系统的范围内以确保生存。
