Ichikawa Masako, Kato Norio, Toda Erika, Kashihara Masakazu, Ishida Yuji, Hiei Yukoh, Isobe Sachiko N, Shirasawa Kenta, Hirakawa Hideki, Okamoto Takashi, Komari Toshihiko
Plant Innovation Center, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan.
Agri-Bio Research Center, KANEKA CORPORATION, 700 Higashibara, Iwata, Shizuoka 438-0802, Japan.
Breed Sci. 2023 Jun;73(3):349-353. doi: 10.1270/jsbbs.22100. Epub 2023 Jun 15.
Somaclonal variation was studied by whole-genome sequencing in rice plants ( L., 'Nipponbare') regenerated from the zygotes, mature embryos, and immature embryos of a single mother plant. The mother plant and its seed-propagated progeny were also sequenced. A total of 338 variants of the mother plant sequence were detected in the progeny, and mean values ranged from 9.0 of the seed-propagated plants to 37.4 of regenerants from mature embryos. The natural mutation rate of 1.2 × 10 calculated using the variants in the seed-propagated plants was consistent with the values reported previously. The ratio of single nucleotide variants (SNVs) among the variants in the seed-propagated plants was 91.1%, which is higher than 56.1% previously reported, and not significantly different from those in the regenerants. Overall, the ratio of transitions to transversions of SNVs was lower in the regenerants as shown previously. Plants regenerated from mature embryos had significantly more variants than different progeny types. Therefore, using zygotes and immature embryos can reduce somaclonal variation during the genetic manipulation of rice.
通过对来自单一母本植物的合子、成熟胚和未成熟胚再生的水稻植株（L.，‘日本晴’）进行全基因组测序，研究了体细胞克隆变异。还对母本植物及其种子繁殖的后代进行了测序。在后代中总共检测到338个母本植物序列变异，平均值范围从种子繁殖植株的9.0到成熟胚再生植株的37.4。利用种子繁殖植株中的变异计算出的自然突变率为1.2×10，与先前报道的值一致。种子繁殖植株变异中单核苷酸变异（SNV）的比例为91.1%，高于先前报道的56.1%，且与再生植株中的比例无显著差异。总体而言，如先前所示，再生植株中SNV的转换与颠换比例较低。从成熟胚再生的植株比不同后代类型具有显著更多的变异。因此，使用合子和未成熟胚可以减少水稻遗传操作过程中的体细胞克隆变异。
