金黄色葡萄球菌表皮溶解毒素A基因的核苷酸序列
Nucleotide sequence of the epidermolytic toxin A gene of Staphylococcus aureus.
作者信息
O'Toole P W, Foster T J
出版信息
J Bacteriol. 1987 Sep;169(9):3910-5. doi: 10.1128/jb.169.9.3910-3915.1987.
Abstract
The nucleotide sequence of the eta gene, which codes for the epidermolytic toxin serotype A of Staphylococcus aureus TC16, is reported. The coding sequence of 840 nucleotides specifies a protein which, when secreted, has a predicted molecular weight of 26,950. The sequence of eta and the deduced amino acid sequence of the toxin have been compared with those of epidermolytic toxin serotype B. The coding sequences have 52% identical residues, and the polypeptides have 40% identical residues. Amino acid residues have been conserved in the areas of the proteins which correspond to major hydrophobic domains, whereas the regions likely to specify antigenic determinants occur in hydrophilic sequences that have diverged. The level of expression of epidermolytic toxin A in S. aureus 8325-4 was shown to be dependent on the integrity of a regulatory gene called agr.
摘要
已报道了编码金黄色葡萄球菌TC16 A型表皮溶解毒素的eta基因的核苷酸序列。840个核苷酸的编码序列指定了一种蛋白质，该蛋白质分泌时预测分子量为26,950。已将eta序列和推导的毒素氨基酸序列与B型表皮溶解毒素的序列进行了比较。编码序列有52%的相同残基，多肽有40%的相同残基。氨基酸残基在与主要疏水结构域相对应的蛋白质区域中保守，而可能指定抗原决定簇的区域出现在已分化的亲水性序列中。已表明金黄色葡萄球菌8325-4中表皮溶解毒素A的表达水平取决于一个名为agr的调控基因的完整性。
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