Departments of Thoracic Surgery.
Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
J Immunother. 2019 Jan;42(1):23-28. doi: 10.1097/CJI.0000000000000249.
Our study was to evaluate the concordance of programmed cell death-ligand 1 (PD-L1) expression between 22C3 and SP263 assay and explore the association of clinicopathologic features with expression of PD-L1 on both tumor cells (TC) and tumor-infiltrating immune cells (IC). We retrospectively assessed the PD-L1 expression in 305 patients with lung adenocarcinoma or adenosquamous carcinoma by 22C3 and SP263 assay. The association of PD-L1 expression by 22C3 assay with clinicopathologic features was also analyzed. The prevalence of PD-L1 expression by 22C3 assay was 20.7% with a ≥50% cutoff and 46.6% with a ≥1% cutoff. The concordance rates between 2 PD-L1 assays while using 1%, 5%, 25%, and 50% positive TC as the cutoffs were 91.8%, 93.1%, 95.1% and 99.0%, respectively. For PD-L1 expression on IC, the concordance rate was 93.4% using a 1% cutoff. According to the results of 22C3 assay, high PD-L1 expression (using a ≥50% cutoff) on TC was significantly associated with smoking, advanced stage disease, and KRAS mutation. PD-L1 expression on IC was significantly associated with smoking and KRAS mutation. PD-L1 expression on TC and IC were both significantly associated with average number of cigarettes smoked ≥20 per day. The 22C3 and SP263 assays were highly concordant for assessment of PD-L1 expression on TC and IC. Patients with KRAS mutation and smoking history, particularly those having a large number of cigarettes smoked per day, were more likely to have PD-L1 expression on both TC and IC.
我们的研究旨在评估 PD-L1 表达在 22C3 和 SP263 检测中的一致性，并探讨 PD-L1 在肿瘤细胞（TC）和肿瘤浸润免疫细胞（IC）上的表达与临床病理特征之间的关联。我们回顾性评估了 305 例肺腺癌或腺鳞癌患者的 PD-L1 表达情况，分别采用 22C3 和 SP263 检测。还分析了 22C3 检测 PD-L1 表达与临床病理特征的关系。采用 22C3 检测时，PD-L1 表达阳性的比例分别为 20.7%（以≥50%为截断值）和 46.6%（以≥1%为截断值）。在分别以 1%、5%、25%和 50%阳性 TC 作为截断值时，两种 PD-L1 检测方法之间的一致性率分别为 91.8%、93.1%、95.1%和 99.0%。对于 IC 上的 PD-L1 表达，使用 1%的截断值时，一致性率为 93.4%。根据 22C3 检测结果，TC 上高 PD-L1 表达（采用≥50%截断值）与吸烟、晚期疾病和 KRAS 突变显著相关。IC 上的 PD-L1 表达与吸烟和 KRAS 突变显著相关。TC 和 IC 上的 PD-L1 表达均与每天吸烟≥20 支的平均支数显著相关。22C3 和 SP263 检测在评估 TC 和 IC 上的 PD-L1 表达方面高度一致。KRAS 突变和吸烟史的患者，尤其是每天吸烟量大的患者，TC 和 IC 上都更有可能表达 PD-L1。
