Shin Eunah, Kim Hye Min, Koo Ja Seung
Department of Pathology, Yonsei University College of Medicine, Seoul, South Korea.
Breast Cancer Res Treat. 2025 Jan;209(2):261-273. doi: 10.1007/s10549-024-07487-4. Epub 2024 Sep 6.
PD-L1 expression and tumor-associated macrophage (TAM) status in phyllodes tumors (PT) have only been examined in a limited number of studies. This study aimed to investigate the expression of PD-L1 and TAM in breast PT and examine their implications.
Tissue microarrays were constructed from 181 PT samples, and immunohistochemistry for PD-L1 antibodies (SP142, SP263, and 22C3) and TAM markers (CD68 and CD163) were performed. The staining results were compared and analyzed with clinicopathological parameters.
Of the 181 samples, 149 were benign, 27 were borderline, and five were malignant. The number of CD68- and/or CD163-positive TAMs increased with increasing PT grades (P < 0.001), and the number of CD68-positive TAMs was significantly positively correlated with that of CD163-positive TAMs (R = 0.704, P < 0.001). Some of the CD68- and/or CD163-positive cells exhibited positivity for actin staining, displaying hybrid characteristics that resemble both histiocytes and myofibroblasts. PD-L1 SP263 tumor cells and PD-L1 SP263 immune cells were the most expressed in malignant PTs (P < 0.001). The number of CD68- and/or CD163-positive TAMs increased when PD-L1 SP263 immune cells were expressed (P < 0.001). The number of CD68- and/or CD163-positive TAMs was positively correlated with PD-L1 22C3 immune cells (R = 0.299, P < 0.001 and R = 0.336, P < 0.001, respectively). Univariate analysis showed that PD-L1 SP263 immune cell expression (P = 0.016) was associated with shorter disease-free survival and that PD-L1 22C3 tumor cell expression (P < 0.001) was associated with shorter overall survival.
The number of CD68- and/or CD163-positive cells increases with increasing PT histological grade, and these cells exhibit hybrid characteristics, resembling both histiocyte and myofibroblasts.
叶状肿瘤（PT）中程序性死亡配体1（PD-L1）的表达及肿瘤相关巨噬细胞（TAM）状态仅在少数研究中被检测。本研究旨在调查乳腺PT中PD-L1和TAM的表达情况并探讨其意义。
用181例PT样本构建组织微阵列，并对PD-L1抗体（SP142、SP263和22C3）和TAM标志物（CD68和CD163）进行免疫组织化学检测。将染色结果与临床病理参数进行比较和分析。
181例样本中，149例为良性，27例为交界性，5例为恶性。CD68和/或CD163阳性TAM的数量随PT分级增加而增多（P<0.001），CD68阳性TAM的数量与CD163阳性TAM的数量呈显著正相关（R=0.704，P<0.001）。部分CD68和/或CD163阳性细胞肌动蛋白染色呈阳性，表现出类似组织细胞和成肌纤维细胞的混合特征。PD-L1 SP263肿瘤细胞和PD-L1 SP263免疫细胞在恶性PT中表达最多（P<0.001）。当PD-L1 SP263免疫细胞表达时，CD68和/或CD163阳性TAM的数量增加（P<0.001）。CD68和/或CD163阳性TAM的数量与PD-L1 22C3免疫细胞呈正相关（R分别为0.299，P<0.001和R=0.336，P<0.001）。单因素分析显示，PD-L1 SP263免疫细胞表达（P=0.016）与无病生存期较短相关，PD-L1 22C3肿瘤细胞表达（P<0.001）与总生存期较短相关。
CD68和/或CD163阳性细胞的数量随PT组织学分级增加而增多，且这些细胞表现出类似组织细胞和成肌纤维细胞的混合特征。
