使用经木瓜蛋白酶处理的小鼠红细胞形成玫瑰花结的方法对人淋巴细胞进行分级分离。
Fractionation of human lymphocytes using rosette formation with papain-treated mouse erythrocytes.
作者信息
Zola H
出版信息
J Immunol Methods. 1977;18(3-4):387-9. doi: 10.1016/0022-1759(77)90193-4.
PMID:304074
Abstract
Human B lymphocytes from rosettes with mouse erythrocytes. If the erythrocytes are pretreated with papain the rosettes are sufficiently stable to allow separation of T and B lymphocytes. The technique is complementary to the sheep erythrocyte rosette method, and gives cell populations with a similar degree of purity.
摘要
人B淋巴细胞与小鼠红细胞形成玫瑰花结。如果红细胞用木瓜蛋白酶预处理，玫瑰花结就足够稳定，能够分离T淋巴细胞和B淋巴细胞。该技术是对绵羊红细胞玫瑰花结法的补充，并且能得到纯度相似的细胞群体。
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