与小鼠红细胞形成玫瑰花结。II. 人类B淋巴细胞和非T淋巴细胞的一种标志物。
Rosette-formation with mouse erythrocytes. II. A marker for human B and non-T lymphocytes.
作者信息
Gupta S, Good R A, Siegal F P
出版信息
Clin Exp Immunol. 1976 Aug;25(2):319-27.
Peripheral blood lymphocytes from healthy control subjects were studied for spontaneous rosette-formation with mouse erythrocytes. The mean percentage of mouse erythrocyte rosette-forming cells (MRFC) in sixty-three subjects was 7.4X3.5; after neuraminidase treatment olymphocytes the mean percentage of mouse erythrocyte rosette-forming cells(NMRFC) was 17.2X5.6. Thymus cell suspensions made only occasional rosettes with mouse erythrocytes. There was no effect of neuraminidase treatment of thymocytes on mouse erythrocyte rosette-formation. Double labelling experiments with other cell surface markers confirmed this binding of mouse erythrocytes to be a B-cell characteristic. Monocytes and granulocytes do not bind mouse erythrocytes. It appears that there is a distinct receptor for the binding of mouse erythrocytes on the surface of lymphocytes carrying surface immunoglobulins; neuraminidase treatment of lymphocytes permits this binding to occur also on virtually all the non-T cells B cells plus the third population cells).
对健康对照受试者的外周血淋巴细胞进行了研究，以观察其与小鼠红细胞的自发玫瑰花结形成情况。63名受试者中，小鼠红细胞玫瑰花结形成细胞（MRFC）的平均百分比为7.4×3.5；用神经氨酸酶处理淋巴细胞后，小鼠红细胞玫瑰花结形成细胞（NMRFC）的平均百分比为17.2×5.6。胸腺细胞悬液与小鼠红细胞仅偶尔形成玫瑰花结。用神经氨酸酶处理胸腺细胞对小鼠红细胞玫瑰花结形成没有影响。用其他细胞表面标志物进行的双重标记实验证实，这种小鼠红细胞的结合是B细胞的特征。单核细胞和粒细胞不结合小鼠红细胞。看来，在携带表面免疫球蛋白的淋巴细胞表面存在一种与小鼠红细胞结合的独特受体；用神经氨酸酶处理淋巴细胞后，这种结合实际上也能在所有非T细胞（B细胞加第三群细胞）上发生。
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