Department of Chemistry , Indian Institute of Technology Guwahati , Guwahati 781039 , Assam , India.
Malaria Research Group, Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati 781039 Assam , India.
Inorg Chem. 2018 Dec 3;57(23):14574-14581. doi: 10.1021/acs.inorgchem.8b02240. Epub 2018 Nov 8.
A new boronic acid functionalized Zr(IV) metal-organic framework having the capability of sensing HO in live cells is reported. The Zr-MOF bears a UiO-66 structure and contains 2-boronobenzene-1,4-dicarboxylic acid (BDC-B(OH)) as a framework linker. The activated Zr-UiO-66-B(OH) compound (called 1') is highly selective for the fluorogenic detection of HO in HEPES buffer at pH 7.4, even in the presence of interfering ROS (ROS = reactive oxygen species) and other biologically relevant analytes. The fluorescent probe was found to display extraordinary sensitivity for HO (detection limit 0.015 μM) in HEPES buffer, which represents a lower value in comparison to those of the MOF probes documented so far for sensing HO using other analytical methods. Taking advantage of its high selectivity and sensitivity for HO in HEPES buffer, the probe was successfully employed for the imaging of intracellular HO. Imaging studies with MDAMB-231 cells revealed the emergence of bright blue fluorescence after loading with probe 1' and subsequent treatment with HO solution.
报道了一种新的硼酸功能化的 Zr(IV) 金属有机骨架，具有在活细胞中感应 HO 的能力。Zr-MOF 具有 UiO-66 结构，包含 2-硼苯-1,4-二羧酸（BDC-B(OH)）作为骨架连接体。活化的 Zr-UiO-66-B(OH)化合物（称为 1'）在 pH 7.4 的 HEPES 缓冲液中对 HO 的荧光检测具有高度选择性，即使存在干扰的 ROS（ROS = 活性氧）和其他生物相关分析物也是如此。荧光探针在 HEPES 缓冲液中对 HO 显示出非凡的灵敏度（检测限 0.015 μM），与迄今为止使用其他分析方法检测 HO 的 MOF 探针相比，这个值更低。利用其在 HEPES 缓冲液中对 HO 的高选择性和灵敏度，该探针成功用于细胞内 HO 的成像研究。用探针 1'负载并随后用 HO 溶液处理 MDAMB-231 细胞后的成像研究显示出明亮的蓝色荧光的出现。
