Zhang Shidong, Yang Feng, Oguejiofor Chike F, Wang Dongsheng, Dong Shuwei, Yan Zuoting
Chinese Academy of Agricultural Sciences, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Lanzhou 730050, China.
Chinese Academy of Agricultural Sciences, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Lanzhou 730050, China.
Res Vet Sci. 2018 Dec;121:130-133. doi: 10.1016/j.rvsc.2018.10.015. Epub 2018 Oct 30.
This study investigated the correlation between endometrial local expressions of serum amyloid A (SAA) and haptoglobin (Hp) and severity of endometritis in 20 Chinese Holstein dairy cows at 40-60 days postpertum, aged 3-6, and 2-5 parities. Endometrial biopsies were collected and categorized into normal, mild endometritis, and severe endometritis based on clinical signs and histopathology. The protein and mRNA expression of SAA and Hp in endometrial tissue were measured using western blot and real-time PCR, respectively. Furthermore, bovine endometrial epithelial cells were isolated and cultured in vitro. The protein and mRNA expression of SAA and Hp were also determined in the cells treated with increasing concentrations of lipopolysaccharide (LPS; 0, 1, 5, 10 μg/ml). The results showed that both of protein and mRNA of SAA was increased in endometritis or in LPS-stimulated cells, and the increases were positively correlated with severity of endometritis in vivo or LPS stimulation strength in vitro. Meanwhile, protein expression of Hp were also increased in endometritis or in LPS-stimulated cells, but the increases were negatively correlative with severity of inflammation as well as mRNA expression of Hp in vivo or in vitro. Therefore, our results suggest that endometrial local expression of SAA is more significant than Hp as a potential biomarker to assess severity of endometritis in cows.
本研究调查了20头产后40 - 60天、年龄3 - 6岁、产次2 - 5胎的中国荷斯坦奶牛子宫内膜中血清淀粉样蛋白A（SAA）和触珠蛋白（Hp）的局部表达与子宫内膜炎严重程度之间的相关性。采集子宫内膜活检样本，并根据临床症状和组织病理学将其分为正常、轻度子宫内膜炎和重度子宫内膜炎。分别采用蛋白质印迹法和实时荧光定量PCR法检测子宫内膜组织中SAA和Hp的蛋白质及mRNA表达。此外，分离并体外培养牛子宫内膜上皮细胞。用不同浓度（0、1、5、10μg/ml）的脂多糖（LPS）处理细胞，检测细胞中SAA和Hp的蛋白质及mRNA表达。结果显示，在子宫内膜炎或LPS刺激的细胞中，SAA的蛋白质和mRNA均升高，且升高程度与体内子宫内膜炎严重程度或体外LPS刺激强度呈正相关。同时，在子宫内膜炎或LPS刺激的细胞中，Hp的蛋白质表达也升高，但其升高程度与体内外炎症严重程度以及Hp的mRNA表达呈负相关。因此，我们的结果表明，作为评估奶牛子宫内膜炎严重程度的潜在生物标志物，子宫内膜局部SAA表达比Hp更具意义。
