Lewis White P, Barnes Rosemary A
NPHS, Cardiff.
Department of Medical Microbiology, University Hospital of Wales, Cardiff, UK.
Med Mycol. 2006 Sep 1;44(Supplement_1):S191-S198. doi: 10.1080/13693780600898003.
PCR is a useful tool to aid in the diagnosis of invasive aspergillosis. However, it is essential that an optimal method be agreed to allow inclusion in future consensus diagnosis criteria. It should be used in conjunction with other methods (e.g., galatomannan (GM) ELISA and high resolution computed tomography (HRCT)) to enhance the opportunity for detection of this devastating infection. This manuscript will try to highlight the benefits but mainly the limitations occurring throughout the process of molecular testing. It will focus on real-time methods although many of the points will be relevant to block-based amplification.
聚合酶链反应（PCR）是辅助诊断侵袭性曲霉病的一项有用工具。然而，必须商定一种最佳方法，以便纳入未来的共识诊断标准。它应与其他方法（如半乳甘露聚糖（GM）酶联免疫吸附测定和高分辨率计算机断层扫描（HRCT））联合使用，以增加检测这种严重感染的机会。本手稿将试图突出分子检测全过程中的益处，但主要是局限性。尽管许多要点与基于扩增块的方法相关，但它将重点关注实时方法。
