Soike K F, Keller P M, Ellis R W
J Med Virol. 1987 Aug;22(4):307-13. doi: 10.1002/jmv.1890220403.
African green monkeys (Cercopithecus aethiops) were immunized with three intramuscular injections of gpI, gpII, or gpIII glycoprotein antigens of varicella-zoster virus (VZV). Antibody responses to VZV were determined by enzyme-linked immunosorbent assay (ELISA) and to simian varicella virus (SVV) by immunofluorescence and by serum neutralization assays. Two weeks following the third immunization with VZV glycoproteins, the monkeys were challenged by inoculation of SVV. Antibodies to gpII or gpIII partially prevented infection by SVV, while the presence of antibodies to gpI was ineffective in preventing disease induced by SVV challenge. Factors affecting the immunogenicity of these antigens in this model are discussed.
用三次肌肉注射水痘 - 带状疱疹病毒（VZV）的糖蛋白I（gpI）、糖蛋白II（gpII）或糖蛋白III（gpIII）抗原对非洲绿猴（猕猴属埃塞俄比亚种）进行免疫。通过酶联免疫吸附测定（ELISA）确定对VZV的抗体反应，通过免疫荧光和血清中和试验确定对猴水痘病毒（SVV）的抗体反应。在用VZV糖蛋白进行第三次免疫两周后，通过接种SVV对猴子进行攻击。针对gpII或gpIII的抗体部分预防了SVV感染，而针对gpI的抗体的存在在预防SVV攻击诱导的疾病方面无效。讨论了在该模型中影响这些抗原免疫原性的因素。
