Aksamit A J, Major E O, Ghatak N R, Sidhu G S, Parisi J E, Guccion J G
J Neuropathol Exp Neurol. 1987 Sep;46(5):556-66. doi: 10.1097/00005072-198709000-00005.
DNA in situ hybridization using a cloned JC virus (JCV) DNA probe labeled with biotin confirmed the presence of JCV DNA in formalin-fixed, paraffin-embedded brain biopsies from four cases of progressive multifocal leukoencephalopathy (PML). Only small pieces of tissue were available in each case. Detection of the JC DNA:DNA hybrids was carried out by affinity cytochemistry. JCV DNA was identified predominantly in the nuclei of interfascicular oligodendrocytes in demyelinated areas of the biopsies. JC virus was isolated from one case, and the diagnosis of PML was substantiated in all cases by electron microscopic identification or immunocytochemical labeling of JC viral antigen. In situ hybridization using a biotin labeled JCV DNA probe is a specific, sensitive and convenient method for confirming the diagnosis of PML in suspected cases evaluated by brain biopsy.
采用生物素标记的克隆型JC病毒（JCV）DNA探针进行DNA原位杂交，证实了4例进行性多灶性白质脑病（PML）患者经福尔马林固定、石蜡包埋的脑活检组织中存在JCV DNA。每例仅获得少量组织。通过亲和细胞化学检测JC DNA:DNA杂交体。JCV DNA主要在活检组织脱髓鞘区域的束间少突胶质细胞核中被鉴定到。从1例患者中分离出JC病毒，所有病例均通过JC病毒抗原的电子显微镜鉴定或免疫细胞化学标记证实为PML。使用生物素标记的JCV DNA探针进行原位杂交是一种用于在通过脑活检评估的疑似病例中确诊PML的特异性、灵敏且便捷的方法。
