Cellular Oncology Laboratory, Department of Experimental Medicine (DIMES), University of Genova, Largo Rosanna Benzi 10, 16132, Genova, Italy.
U.O. Molecular Pathology, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi 10, 16132, Genova, Italy.
Stem Cell Res Ther. 2018 Nov 8;9(1):300. doi: 10.1186/s13287-018-1056-1.
Restoration of damaged tissues through the activation of endogenous progenitors is an attractive therapeutic option. A deep evaluation of the intrinsic stem/progenitor cell properties as well as the reciprocal interactions with injured environments is of critical importance.
Here, we show that bone marrow stromal cell antigen 2 (BST2) allows the isolation of a population of circulating progenitors, the circulating healing (CH) cells, characterized by a distinctive core signature. The bone marrow (BM) origin of BST2 CH cells has been strengthened by the co-expression of leptin receptor, the hallmark of a subpopulation of BM-skeletal stem cells.
BST2 CH cells retained the capacity to (i) respond to injury signals generated by a bone fracture, (ii) modify the expression of cell motility genes following damage, and (iii) react to hepatocyte growth factor-activator (HGFA), an injury-related stimulus sufficient to induce their transition into G, a state in which cells are functionally activated and participate in tissue repair.
Taken together, these results could pave the way for the identification of new strategies to enhance and potentiate endogenous regenerative mechanisms for future therapies.
通过激活内源性祖细胞来修复受损组织是一种有吸引力的治疗选择。深入评估内在的干细胞/祖细胞特性以及与受损环境的相互作用至关重要。
在这里，我们表明骨髓基质细胞抗原 2（BST2）允许分离出一群循环祖细胞，即循环修复（CH）细胞，其特征是具有独特的核心特征。BST2 CH 细胞的骨髓（BM）起源已通过瘦素受体的共表达得到加强，瘦素受体是 BM-骨骼干细胞亚群的标志。
BST2 CH 细胞保留了以下能力：（i）对骨骨折产生的损伤信号做出反应；（ii）在损伤后改变细胞迁移基因的表达；（iii）对肝细胞生长因子激活剂（HGFA）做出反应，HGFA 是一种与损伤相关的刺激物，足以诱导它们进入 G 期，在 G 期细胞功能激活并参与组织修复。
综上所述，这些结果可能为未来治疗确定增强和增强内源性再生机制的新策略铺平道路。
