Martínez-Salazar María Fernanda, Soriano-Martínez María de la Luz, Juantorena-Ugas Alina, Almenares-López Damianys, Yescas Petra, Boll Marie-Catherine, Monroy-Noyola Antonio
Laboratorio de Bioquímica, Facultad de Ciencias del Deporte, Universidad Autónoma del Estado de Morelos. Cuernavaca, México.
Laboratorio de Neuroprotección, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos. Cuernavaca, México.
Colomb Med (Cali). 2018 Sep 30;49(3):223-227. doi: 10.25100/cm.v49i2.2217.
The serum paraoxonase-1 (PON1) associated to HDL presents two common polymorphisms in the positions 192 and 55. These polymorphisms are considered determinant of the capacity of HDL to protect LDL from their oxidative modification. In this context, the PON1 genotype has been associated with cardiovascular diseases, including stroke.
To determine the allelic and genotypic frequencies of PON1 L55M and Q192R as well as the enzymatic activities of PON1 in subjects with and without atherothrombotic stroke.
There were included 28 people with atherothrombotic stroke and 29 without stroke. The genotyping was carried out by PCR-RFLP and the phenotyping by measurement of the activities of paraoxonase and arylesterase in serum.
For the polymorphism Q192R, the allelic frequencies (Q/R) were 0.46/0.54 and 0.48/0.52 (= 0.843) for the control group and the group with stroke, respectively. While for the polymorphism L55M, the allelic frequencies (L/M) were 0.81/0.19 for the control group, and 0.78/0.22 for the group with stroke (= 0.610). The activity levels of paraoxonase were not significantly different between the control and stroke groups (450 vs. 348 UI/mL, = 0.093) While the activity levels of arylesterase were significantly different between the studied groups (90 vs. 70 UI/mL, = 0.001); however, upon adjustment by multiple linear regression, it was not longer significant.
The polymorphisms Q192R and L55M, and the paraoxonase activity of PON1 are not risk factors for atherothrombotic stroke according to the results of this study.
与高密度脂蛋白（HDL）相关的血清对氧磷酶-1（PON1）在第192位和第55位存在两种常见的多态性。这些多态性被认为是HDL保护低密度脂蛋白（LDL）免受氧化修饰能力的决定因素。在这种情况下，PON1基因型与包括中风在内的心血管疾病有关。
确定有和没有动脉粥样硬化血栓形成性中风的受试者中PON1 L55M和Q192R的等位基因和基因型频率以及PON1的酶活性。
纳入28例动脉粥样硬化血栓形成性中风患者和29例无中风患者。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行基因分型，通过测量血清中对氧磷酶和芳基酯酶的活性进行表型分析。
对于Q192R多态性，对照组和中风组的等位基因频率（Q/R）分别为0.46/0.54和0.48/0.52（P = 0.843）。而对于L55M多态性，对照组的等位基因频率（L/M）为0.81/0.19，中风组为0.78/0.22（P = 0.610）。对照组和中风组之间对氧磷酶的活性水平无显著差异（450对348国际单位/毫升，P = 0.093），而芳基酯酶的活性水平在研究组之间有显著差异（90对70国际单位/毫升，P = 0.001）；然而，经多元线性回归调整后，差异不再显著。
根据本研究结果，Q192R和L55M多态性以及PON1的对氧磷酶活性不是动脉粥样硬化血栓形成性中风的危险因素。
