Laboratory of Biochemistry-Toxicology, Monastir University Hospital, 5000 Monastir, Tunisia.
Environ Health Prev Med. 2012 Jul;17(4):316-21. doi: 10.1007/s12199-011-0256-4. Epub 2011 Dec 29.
This study aims to investigate the effect of cigarette smoking on paraoxonase 1 (PON1) activity according to PON1 L55M and PON1 Q192R gene polymorphisms.
Our sample included 300 voluntary subjects: 138 nonsmokers and 162 current smokers aged 38.47 ± 21.91 and 35.55 ± 16.03 years, respectively. PON1 activity was determined by kinetic methods. L55M and Q192R gene polymorphisms of PON1 were determined by multiplex polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP).
We found in smokers a significant decrease of PON1 activity before and after adjustment. We noted a significant association between smoking status and lower PON1 activity [odds ratio (OR) = 3.03, confidence interval 95% = 1.5-5.9, p = 0.001]. In smokers, there was significant association between PON1 activity and PON1 L55M polymorphisms (p = 0.01). Also, the 55MM genotype presented the lowest paraoxonase activity, while the 55LL genotype showed the highest one. After adjustment for confounding variables, smokers with PON1 L55M polymorphism had the highest risk for lower PON1 activity; however, PON1 Q192R genotype might protect smokers from decrease in PON1 activity. We found significant interaction between the effect of cigarette smoking and both PON1 L55M and PON1 Q192R polymorphisms on lower PON1 activity.
Cigarette smoking was significantly associated with decrease in PON1 activity. Moreover, PON1 L55M polymorphism predisposes smokers to decreased PON1 activity in contrast to PON1 Q192R genotype.
本研究旨在探讨根据对氧磷酶 1（PON1）L55M 和 Q192R 基因多态性，吸烟对 PON1 活性的影响。
我们的样本包括 300 名自愿受试者：138 名不吸烟者和 162 名当前吸烟者，年龄分别为 38.47±21.91 岁和 35.55±16.03 岁。通过动力学方法测定 PON1 活性。通过多重聚合酶链反应（PCR）限制性片段长度多态性（RFLP）测定 PON1 的 L55M 和 Q192R 基因多态性。
我们发现吸烟者的 PON1 活性在调整前后均显著降低。我们注意到吸烟状态与较低的 PON1 活性之间存在显著关联[比值比（OR）=3.03，95%置信区间=1.5-5.9，p=0.001]。在吸烟者中，PON1 活性与 PON1 L55M 多态性之间存在显著关联（p=0.01）。此外，55MM 基因型表现出最低的对氧磷酶活性，而 55LL 基因型表现出最高的活性。在调整混杂变量后，携带 PON1 L55M 多态性的吸烟者发生较低 PON1 活性的风险最高；然而，PON1 Q192R 基因型可能使吸烟者免于 PON1 活性降低。我们发现吸烟对 PON1 活性的影响与 PON1 L55M 和 PON1 Q192R 多态性之间存在显著交互作用。
吸烟与 PON1 活性降低显著相关。此外，与 PON1 Q192R 基因型相反，PON1 L55M 多态性使吸烟者更容易发生 PON1 活性降低。
